Calcium- and barium-dependent extracellular alkaline shifts evoked by electrical activity in rat hippocampal slices

Neuroscience
I I Grichtchenko, Mitchell Chesler

Abstract

Synaptic activation of central neurons has been associated with rapid extracellular alkalinization. In this report, we directly activated CA1 pyramidal cells by antidromic invasion, or by field stimulation. Antidromic activation produced no pH change, despite a robust population spike in five of 11 slices. In six slices, antidromic stimulation at 10 Hz evoked a small alkalinization in stratum pyramidale (0.04 +/- 0.01 unit pH) which grew to 0.10-0.20 unit pH at 50-100 Hz, and was blocked in 0 Ca2+ media. Simultaneous pH recordings revealed no alkalinizations in stratum radiatum, despite robust alkaline shifts in stratum pyramidale. When synaptic transmission was blocked by 6-cyano-7-nitroquinoxaline-2,3-dione, DL-2-amino-5-phosphonovalerate and picrotoxin, the Schaffer collateral-induced alkaline shift in stratum radiatum was abolished. With adequate stimulus strength and orientation, however, alkaline shifts in stratum radiatum could still be elicited, presumably by direct activation of the CA1 population. The non-synaptic alkaline shifts ranged from 0.10-0.20 unit pH, were amplified by benzolamide, and blocked by tetrodotoxin, 0 Ca2+ saline, and 300-400 microM Cd2+. Although directly activated alkaline shifts were never obser...Continue Reading

References

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Citations

Mar 10, 2001·Progress in Neurobiology·C Eder, T E DeCoursey
Nov 27, 2009·Journal of Neurophysiology·Sachin Makani, Mitchell Chesler
Oct 17, 2002·The Journal of Physiology·Debbie Willoughby, Christof J Schwiening
Apr 14, 2006·Journal of Neurophysiology·Chi-Kun TongMitchell Chesler
Sep 25, 2003·Physiological Reviews·Mitchell Chesler

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