PMID: 2117985Jun 4, 1990Paper

Calcium- and voltage-dependent potassium channel in the rat retinal amacrine cells identified in vitro using a cell type-specific monoclonal antibody

Brain Research
K AkagawaK Uyemura

Abstract

Single-channel currents were studied from identified amacrine cells of rat retinal monolayer cultures. Cells were immunohistochemically identified using an amacrine cell-specific monoclonal antibody, HPC-1. Single-channel currents with the following properties were observed. (1) The mean opening duration was voltage dependent, tending to increase on hyperpolarization. (2) The reversal potential was almost identical to the calculated EK derived from the potassium concentration gradient. (3) The conductance was 110 pS at a low extracellular potassium concentration. (4) Currents were blocked by TEA. (5) Mean opening duration was also dependent on the calcium concentration of the intracellular side. These properties were consistent with the voltage and calcium-dependent potassium channels of a large conductance. Our results also demonstrated that cell-type-specific monoclonal antibodies together with electrophysiological recordings offer a very useful method to investigate the physiological properties of distinct neuronal types in mixed cell cultures.

References

Dec 1, 1987·Pflügers Archiv : European journal of physiology·N Quartararo, P H Barry
Sep 1, 1988·Trends in Neurosciences·R H Masland
Jun 1, 1985·Brain Research·C J BarnstableK Akagawa
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·C J BarnstableJ P Horn

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Citations

Nov 1, 1993·Journal of Neurocytology·S KohY Tashiro
Dec 1, 1990·Brain Research. Developmental Brain Research·K Akagawa
Oct 27, 1995·Brain Research. Developmental Brain Research·M ArakiH Kimura
Jul 16, 2008·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·M SozmenS Dag Erginsoy

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