Calcium-activated chloride channels clamp odor-evoked spike activity in olfactory receptor neurons

Scientific Reports
Joseph D ZakVenkatesh N Murthy

Abstract

The calcium-activated chloride channel anoctamin-2 (Ano2) is thought to amplify transduction currents in olfactory receptor neurons (ORNs), a hypothesis supported by previous studies in dissociated neurons from Ano2-/- mice. Paradoxically, despite a reduction in transduction currents in Ano2-/- ORNs, their spike output for odor stimuli may be higher. We examined the role of Ano2 in ORNs in their native environment in freely breathing mice by imaging activity in ORN axons as they arrive in the olfactory bulb glomeruli. Odor-evoked responses in ORN axons of Ano2-/- animals were consistently larger for a variety of odorants and concentrations. In an open arena, Ano2-/- animals took longer to approach a localized odor source than Ano2+/+ animals, revealing clear olfactory behavioral deficits. Our studies provide the first in vivo evidence toward an alternative or additional role for Ano2 in the olfactory transduction cascade, where it may serve as a feedback mechanism to clamp ORN spike output.

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Citations

May 3, 2019·The Journal of General Physiology·Tiago HenriquesSimone Pifferi
Mar 22, 2019·Cancers·Karl KunzelmannRainer Schreiber
Apr 17, 2019·International Journal of Molecular Sciences·Erika Calvo-Ochoa, Christine A Byrd-Jacobs
Jan 16, 2021·Cell and Tissue Research·Anna BoccaccioSimone Pifferi
May 4, 2021·PloS One·Johannes ReisertAlan Gelperin
Jul 14, 2021·Physiological Reviews·Ivan ManziniCorrado Di Natale

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Methods Mentioned

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Software Mentioned

MATLAB
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