PMID: 7545279May 1, 1995Paper

Calcium-activated chloride conductance is not increased in pancreatic duct cells of CF mice

Pflügers Archiv : European journal of physiology
J P WinpennyB E Argent

Abstract

Calcium-activated anion secretion is elevated in the pancreatic ductal epithelium of transgenic cf/cf mice which lack the cystic fibrosis transmembrane conductance regulator (CFTR). To elucidate whether this effect is due to increased activity of calcium-activated chloride channels, we have studied the relationship between CFTR and calcium-activated chloride currents in pancreatic duct cells isolated from Cambridge cf/cf mice. CFTR chloride currents activated by cAMP were detected in 59% (29/49) of wild-type cells and in 50% (20/40) of heterozygous cells. However, we could not detect any CFTR currents in the homozygous cf/cf cells (0/25). The maximum CFTR current density measured at a membrane potential of 60 mV was 23.5 +/- 2.8 pA/pF (n = 29) in wild-type cells, and about half that value, i.e. 12.4 +/- 1.6 pA/pF (n = 20) in heterozygotes (P = 0.004). Calcium-activated chloride currents were detected in 73% (24/33) of wild-type, 75% (21/28) of heterozygous and in 58% (7/12) of homozygous cf/cf cells. There was no significant difference between the steady-state calcium-activated current densities in the three genotypic groups; the current measured at 60 mV being 527 +/- 162 pA/pF (n = 24) from wild-type, 316 +/- 35 pA/pF (n = 21...Continue Reading

References

Sep 12, 1992·Lancet·W H ColledgeM J Evans
Apr 24, 1992·Science·N A BradburyK L Kirk
Aug 21, 1992·Science·J N SnouwaertB H Koller
Nov 1, 1990·The American Journal of Physiology·M A GrayB E Argent
Oct 1, 1988·The Journal of Membrane Biology·M A GrayB E Argent
Feb 1, 1989·The American Journal of Physiology·N J Willumsen, R C Boucher
Aug 1, 1989·The American Journal of Physiology·M A GrayB E Argent
Oct 1, 1986·Quarterly Journal of Experimental Physiology : an International Journal of the Physiological Society·B E ArgentR Green
Oct 1, 1993·Human Molecular Genetics·W K O'NealA Bradley
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·L L ClarkeR C Boucher
Jan 1, 1994·The American Journal of Physiology·M A GrayB E Argent
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·L S PrinceR B Marchase
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·J H PoulsenT E Machen
May 1, 1994·The American Journal of Physiology·B R GrubbR C Boucher
Mar 1, 1993·The American Journal of Physiology·M A GrayB E Argent
May 1, 1993·Nature Genetics·R RatcliffW H Colledge
Apr 1, 1994·Pflügers Archiv : European journal of physiology·L J GaliettaG Romeo

❮ Previous
Next ❯

Citations

Feb 28, 2009·Journal of Physiology and Biochemistry·M GarcíaJ J Calvo
Aug 6, 2009·Pflügers Archiv : European journal of physiology·Patricia PascuaJosé I San Román
Feb 16, 2005·Annual Review of Physiology·Martin C StewardR Maynard Case
Feb 3, 2012·Physiological Reviews·Min Goo LeeShmuel Muallem
Aug 5, 2006·American Journal of Respiratory Cell and Molecular Biology·Claudine GuilbaultDanuta Radzioch
Mar 28, 2008·American Journal of Respiratory Cell and Molecular Biology·Brett M RollinsRobert Tarran
Oct 1, 1996·Molecular Neurobiology·S Basavappa, J C Ellory
Mar 9, 2010·Experimental Lung Research·Malcolm BrodlieChristopher Ward
Feb 25, 2009·The Journal of Physiology·Laura L Marsey, John P Winpenny
Nov 14, 2000·Molecular Genetics and Metabolism·J E Larson, J C Cohen
Mar 15, 2006·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·R Maynard Case
Dec 1, 1995·Bioscience Reports·M A GrayB E Argent
Jun 2, 2016·Histochemistry and Cell Biology·Pauline T IkpaMarcel J C Bijvelds
Feb 27, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Horst FischerJonathan H Widdicombe
Jul 31, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Omar A ItaniMichael J Welsh
Apr 25, 2003·American Journal of Physiology. Cell Physiology·Peying FongMichael A Gray
May 12, 1998·The American Journal of Physiology·R TarranB E Argent
Jan 29, 1999·Physiological Reviews·B R Grubb, R C Boucher
May 2, 2003·American Journal of Physiology. Renal Physiology·Zhiqiang QuH Criss Hartzell
Jan 14, 1999·The American Journal of Physiology·H ShumakerM Soleimani
Feb 1, 2013·American Journal of Physiology. Cell Physiology·Jing WangIvana Novak
Nov 13, 2020·Frontiers in Pharmacology·Julie SchnipperHalima Ouadid-Ahidouch
Jun 29, 2000·The Medical Clinics of North America·M Soleimani, C D Ulrich

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.