Calcium-activated potassium channel gene expression in the midgut of Drosophila

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
R Brenner, Nigel S Atkinson

Abstract

The slowpoke gene of Drosophila encodes a pore-forming subunit of a BK-type Ca(2+)-activated K+ channel. The gene is expressed in neurons, muscles, tracheal cells and in the midgut. The P1 transgene gene contains the entire slowpoke transcriptional control region and drives the expression of a reporter protein comprised of slowpoke amino terminal sequences fused to beta-galactosidase. Here we show that midgut expression is limited to the copper cell and iron cell regions. The copper cell region is composed of two cell types, the copper cells and the interstitial cells. The P1 transgene is expressed in the interstitial cells but not the copper cells. Furthermore, we show that the reporter protein is apically localized in the interstitial cells. In these cells, the slowpoke Ca(2+)-activated K+ channel is thought to participate in the transport of ions between the hemolymph and the lumen of the gut. Subcellularly localized BK channels may be involved in the secretion of acid into the gut lumen. An analogous role for basolaterally localized BK channels has been proposed in the acid-secreting intercalating cells of the human kidney.

References

Jan 1, 1991·Annual Review of Physiology·D C Dawson
Jan 1, 1991·Methods in Enzymology·D M Templeton, M G Cherian
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M J Welsh, J D McCann
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T ElkinsC F Wu
Nov 1, 1994·Brain Research. Molecular Brain Research·S I DworetzkyV K Gribkoff
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·M WallnerL Toro

❮ Previous
Next ❯

Citations

Mar 1, 1997·Invertebrate Neuroscience : in·T ThomasN S Atkinson
May 17, 2011·Invertebrate Neuroscience : in·Hansjürgen Schuppe, Philip L Newland
Nov 1, 2005·Plant Physiology·Yilin LiuKeyan Zhu-Salzman
May 24, 2006·Genes, Brain, and Behavior·J Y YuN S Atkinson
Feb 3, 1999·Annals of the New York Academy of Sciences·N S AtkinsonJ L Wilbur
Mar 10, 2004·BMC Neuroscience·Angela M JaramilloIrwin B Levitan
Oct 19, 2007·PLoS Biology·Yan WangNigel S Atkinson
Jun 9, 2005·Microscopy Research and Technique·Grzegorz TylkoElzbieta Pyza
Mar 28, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xiaoxi WangBing Zhou
Aug 27, 2011·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Lilit AtanesyanWalter Schaffner
Oct 16, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xiaona Tang, Bing Zhou
Oct 6, 2018·Genetics·Irene Miguel-AliagaBruno Lemaitre

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.