PMID: 9160165Apr 1, 1997Paper

Calcium-activated potassium channels in cultured human endothelial cells are not directly modulated by nitric oxide

Cell Calcium
M HaburcákBernd Nilius

Abstract

Nitric oxide has been proposed to directly activated large conductance Ca(2+)-dependent K+ channels (BKCa) [Bolotina V.M., Najibi S., Palacino J.J., Pagano P.J., Cohen R.A. Nitric oxide directly activates calcium-dependent potassium channels in vascular smooth muscle. Nature 1994; 368: 850-853]. The nitric oxide (NO) donor S-nitrosocysteine (SNOC) was used to evaluate a possible direct modulation of BKCa by NO in EAhy926 (EA cells), a cultured human umbilical vein derived endothelial cell line, using the whole-cell, cell-attached and inside-out configuration of the patch-clamp technique, together with simultaneous amperometric measurement of NO and the concentration of free intracellular calcium [Ca2+]i. BKCa channels with a large conductance of approximately 190 pS, voltage-dependent activation and a reversal potential close to -80 mV have been identified in EA cells. Exposure of EA cells in the experimental chamber to 1 mM SNOC delivered approximately 5 microM NO, as recorded by an amperometric probe in situ. SNOC produced a modest increases in [Ca2+]i that was insufficient to activate BKCa channels. NO alone neither activated BKCa channels directly nor modulated preactivated BKCa channels in EA cells. These results do not su...Continue Reading

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Citations

Oct 13, 2007·Naunyn-Schmiedeberg's Archives of Pharmacology·Birgit Eichhorn, Dobromir Dobrev
Mar 28, 2008·Pflügers Archiv : European journal of physiology·Guo Hua LiangSuk Hyo Suh
Apr 20, 2001·The International Journal of Biochemistry & Cell Biology·M FaehlingJ Waltenberger
Mar 1, 2000·British Journal of Pharmacology·D YeD A Mathers
Oct 6, 2001·American Journal of Respiratory Cell and Molecular Biology·A OlschewskiB V Safronov
Feb 6, 2002·The Journal of Biological Chemistry·Hiroyuki WatanabeBernd Nilius
May 5, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Shaun L Sandow, T Hilton Grayson
Jul 27, 2000·Endothelium : Journal of Endothelial Cell Research·S H SuhB Nilius
Oct 3, 2001·Physiological Reviews·B Nilius, G Droogmans
Apr 2, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Piotr BednarczykAdam Szewczyk

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