PMID: 7016117Dec 15, 1980Paper

Calcium-activated thiol-proteinase activity in the fusion of rat erythrocytes induced by benzyl alcohol

The Biochemical Journal
Q F AhkongJ A Lucy

Abstract

1. Rat erythrocytes were fused by incubation with benzyl alcohol and Ca2+. 2. Cell fusion was inhibited by EGTA, N-ethylmaleimide, tetrathionate, iodoacetamide, cystamine, Tos-Lys-CH2Cl, and to a lesser extent by Tos-Phe-CH2Cl. Phenylmethanesulphonyl fluoride, Tos-Arg-OMe and histamine did not inhibit cell fusion. 3. Gel electrophoresis of membrane proteins from "ghosts" of the erythrocytes treated with benzyl alcohol showed that a high-molecular-weight polymer was present: this was consistent with the entry into the cells of Ca2+ and the activation of a transglutaminase enzyme. 4. In the treated cells the proteins corresponding to bands 2 and 3 in human erythrocytes were decreased, and a polypeptide with a slightly greater mobility than band 3 was produced. 5. These changes were inhibited by EGTA, N-ethylmaleimide, tetrathionate, iodoacetamide, cystamine, and Tos-Lys-CH2Cl, but not by phenylmethanesulphonyl fluoride, Tos-Arg-OMe, or histamine. 6. The intramembraneous particles of the P-fracture face of cells treated with benzyl alcohol to induce fusion were decreased in number and were susceptible to cold-induced aggregation; both of these phenomena were markedly inhibited to EGTA, and partially inhibited by Tos-Lys-CH2Cl and ...Continue Reading

Citations

Sep 14, 1981·Life Sciences·S Ishiura
Jul 1, 1981·Experimental Cell Research·H Tolleshaug, T Berg
Apr 7, 1986·FEBS Letters·J A Lucy, Q F Ahkong
Jul 22, 1983·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·M J Whitaker, P F Baker
Sep 1, 1988·Journal of Neurochemistry·K YanagisawaT Miyatake
Jan 1, 1982·The Journal of Membrane Biology·U Zimmermann, J Vienken
May 1, 1989·American Journal of Hematology·N I Swislocki, J M Tierney
Jun 13, 1986·Biochimica Et Biophysica Acta·Q F Ahkong, J A Lucy
Nov 30, 1982·Biochimica Et Biophysica Acta·U Zimmermann
Jan 1, 1984·The International Journal of Biochemistry·S Takehara, H Terayama
Apr 1, 1984·Infection and Immunity·T A CebulaV J Ferrans
Jan 1, 1984·Progress in Neurobiology·U J Zimmerman, W W Schlaepfer
May 16, 1984·Biochemical and Biophysical Research Communications·U J Zimmerman, W W Schlaepfer
Sep 30, 1982·Biochemical and Biophysical Research Communications·L GuenetJ Y Le Gall

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