Calcium alginate gels as stem cell matrix-making paracrine stem cell activity available for enhanced healing after surgery

PloS One
Andreas SchmittJulia Engert

Abstract

Regeneration after surgery can be improved by the administration of anabolic growth factors. However, to locally maintain these factors at the site of regeneration is problematic. The aim of this study was to develop a matrix system containing human mesenchymal stem cells (MSCs) which can be applied to the surgical site and allows the secretion of endogenous healing factors from the cells. Calcium alginate gels were prepared by a combination of internal and external gelation. The gelling behaviour, mechanical stability, surface adhesive properties and injectability of the gels were investigated. The permeability of the gels for growth factors was analysed using bovine serum albumin and lysozyme as model proteins. Human MSCs were isolated, cultivated and seeded into the alginate gels. Cell viability was determined by AlamarBlue assay and fluorescence microscopy. The release of human VEGF and bFGF from the cells was determined using an enzyme-linked immunoassay. Gels with sufficient mechanical properties were prepared which remained injectable through a syringe and solidified in a sufficient time frame after application. Surface adhesion was improved by the addition of polyethylene glycol 300,000 and hyaluronic acid. Humans MSCs ...Continue Reading

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Citations

Jun 10, 2017·Pharmaceutical Development and Technology·Ana Henriques MotaCatarina Pinto Reis
Mar 10, 2018·Bioengineering·Biraja C DashHenry Hsia
Aug 2, 2018·Bioengineering·Chantell FariasMaryam Mobed-Miremadi
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Jul 10, 2020·BioResearch Open Access·Anaïs BarreSophie Le Ricousse
Oct 20, 2020·Advanced Healthcare Materials·Fabio Rizzo, Nermin Seda Kehr
Feb 14, 2021·Materials·Magdalena B ŁabowskaIzabela Michalak
Oct 7, 2019·Biophysical Journal·Megan Davis-FieldsVernita D Gordon
Mar 24, 2018·Advanced Drug Delivery Reviews·Robert DimatteoTatiana Segura
Jul 31, 2021·Journal of Biomaterials Science. Polymer Edition·Samuel Ogbeide Ebhodaghe
Oct 17, 2021·Journal of Tissue Engineering and Regenerative Medicine·Fiona R PassanhaVanessa L S LaPointe

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Methods Mentioned

BETA
ELISA
ABTS
Fluoresence microscopy
transfection

Software Mentioned

TextureExponent32
SPSS

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