PMID: 7581320Sep 1, 1995Paper

Calcium and hydrogen ion concentrations in the parasitophorous vacuoles of epithelial cells infected with the microsporidian Encephalitozoon hellem

The Journal of Eukaryotic Microbiology
G J LeitchS Wallace

Abstract

Microsporidia of the genus Encephalitozoon undergo merogony and sporogony in a parasitophorous vacuole within the host cell. Cultured green monkey kidney cells infected with Encephalitozoon hellem were loaded with the fluorescent dyes fura-2 or BCECF in order to measure intracellular concentrations of calcium and hydrogen ions respectively. Both the parasitophorous vacuole calcium concentration and pH values resembled those of the host cell cytoplasm in infected cells. Calcein entered the parasitophorous vacuole but not other host cell vacuoles or parasite stages within the parasitophorous vacuole. The lack of a pH or calcium concentration gradient across the parasitophorous vacuole membrane and the permeability of this membrane to a large anion such as calcein suggest that the vacuole membrane surrounding E. hellem resembles that surrounding some other intracellular parasites such as Toxoplasma gondii. A potential role is discussed for the parasitophorous vacuole calcium concentration in germination in situ.

References

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Citations

Sep 22, 2001·International Journal for Parasitology·K J Saliba, K Kirk
Apr 25, 2013·Antimicrobial Agents and Chemotherapy·Maria Anete LalloJoão Manoel de Castro
Dec 20, 2000·Antimicrobial Agents and Chemotherapy·G J LeitchG S Visvesvara
Aug 7, 2003·Applied and Environmental Microbiology·Rebecca M HoffmanB Helen Jost
May 10, 2011·International Journal of Medical Microbiology : IJMM·Wolfgang BohneUwe Gross
May 26, 2012·Experimental Parasitology·Maria Anete Lallo, Marisa Porta Miche Hirschfeld
Aug 13, 2010·Gastroentérologie Clinique Et Biologique·S Anane, H Attouchi
Mar 14, 2000·Veterinary Pathology·K Wasson, R L Peper
Jul 27, 2000·American Journal of Physiology. Cell Physiology·M Colden-Stanfield, M Scanlon

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