Calcium and insulin-like growth factor I stimulation of sodium-dependent phosphate transport and proliferation of cultured rat osteoblasts

Biochemical and Biophysical Research Communications
C SchmidJ Zapf

Abstract

Calcium (Ca) stimulates proliferation of osteoblasts in vitro, an effect proposed to be mediated by IGF I. Addition of 1 mM Ca or of 1 nM IGF I to the medium (0.3 mM Ca) of a rat bone-derived cell line, PyMS, stimulated not only DNA synthesis but also sodium-dependent (Nad) phosphate (Pi) uptake, the latter, within 2 h. These cells barely express and produce IGF I. IGF binding protein-3 which inhibits IGF action decreased neither basal nor Ca-stimulated but IGF I-stimulated NadPi transport and DNA synthesis, indicating that Ca stimulated NadPi transport and DNA synthesis independently of IGF I. The effects of Ca and IGF I on DNA synthesis were additive. 1 microM nifedipine blocked IGF I- and Ca-stimulated DNA synthesis but not NadPi transport, suggesting that Ca influx is not mediating the NadPi transport-enhancing IGF I signal but is required for IGF I-induced osteoblast proliferation.

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Citations

Jan 10, 2012·Experimental Physiology·Zanming ShenMonika Schweigel-Röntgen
Aug 3, 2006·The American Journal of Chinese Medicine·Md Rafiqul AlamNam Soo Kim
May 24, 2005·Regulatory Peptides·Christoph SchmidMartina Gosteli-Peter
May 8, 2000·Journal of Neuroscience Research·M C CatlinL G Costa
Sep 25, 2003·Journal of Cellular Biochemistry·George R Beck
Sep 18, 2010·Cellular and Molecular Life Sciences : CMLS·Solmaz KhoshniatLaurent Beck
Dec 8, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ioannis ChristodoulouJulia M Polak
Nov 2, 2013·Clinical Journal of the American Society of Nephrology : CJASN·Ting JiaAbdul Rashid Qureshi
Jan 20, 2012·Endocrine·Evangelos ZoidisChristoph Schmid
Mar 6, 1999·The Journal of Biological Chemistry·Z JobbagyW B Anderson
Apr 16, 2004·Endocrinology·Naibedya ChattopadhyayEdward M Brown
Sep 14, 1999·The American Journal of Physiology·A N Alexander, H V Carey
Feb 24, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·A N Alexander, H V Carey
Jan 16, 1999·Journal of the American Society of Nephrology : JASN·D P QingJ D Kopple

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