PMID: 6985147Dec 1, 1982Paper

Calcium and magnesium binding to thin and thick filaments in skinned muscle fibres: electron probe analysis

Journal of Muscle Research and Cell Motility
T KitazawaA P Somlyo

Abstract

Electron probe analysis of ultrathin cryosections with high spatial resolution was used to determine in situ the concentrations of Ca2+ and Mg2+ bound in the absence of ATP to myofilaments in the I and A-bands of skinned frog skeletal muscle. At 2.2 x 10(-11) M Ca2+ and 2.7 x 10(-9) M Mg2+, the inexchangeably bound Mg2+ in the I-band was equivalent to the amount of divalent cations known to be inexchangeably bound to F-actin, while the Ca2+ bound to the I-band was not significantly above zero. The bound Mg2+ in the I-band was not exchangeable with Ca2+ even when the skinned fibres were exposed to 10 mM Ca2+ solution. These results clearly indicate that Mg2+, rather than Ca2+, is the divalent cation bound to F-actin in the thin filaments in situ. In the presence of 1 mM Mg2+, the exchangeable Ca2+ bound to the I-band was increased as a function of the free Ca2+, while that in the A-band was not significantly changed with [Ca2+] up to 2 x 10(-5) M, and increased to approximately 0.8 mol Ca2+ per mol myosin at 10(-4) M Ca2+. At a saturating free Ca2+ in Tris-Cl solution, the bound Ca2+ content (2-3 mol Ca2+ per mol troponin) of the nonoverlapping I-band was unexpectedly low; the replacement of Tris with Na+ enhanced Ca2+ binding t...Continue Reading

References

Sep 1, 1979·Journal of Biochemistry·J Wikman-Coffelt, S Srivastava
Nov 1, 1979·Archives of Biochemistry and Biophysics·G L AlonsoS Terradas de Fermani
Jul 1, 1976·The Journal of Physiology·J S Gillespie, A T McKnight
Aug 8, 1977·Biochemical and Biophysical Research Communications·J T Stull, C W High
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S M Cohen, C T Burt
Nov 2, 1978·European Journal of Biochemistry·J P van EerdJ F Pechère
Jun 1, 1969·Biochemistry·R HerzI Reiss
Jul 26, 1972·Nature: New Biology·R D Bremel, A Weber
Feb 15, 1974·Journal of Molecular Biology·K Morimoto, W F Harrington
Jan 1, 1969·Journal of Ultrastructure Research·A R Spurr
Jan 1, 1968·Progress in Biophysics and Molecular Biology·S Ebashi, M Endo
May 1, 1968·The Journal of General Physiology·F Fuchs, F N Briggs
Oct 1, 1968·Journal of Biochemistry·S EbashiF Ebashi
Jan 14, 1969·Biochimica Et Biophysica Acta·M Kasai
Feb 1, 1969·The Journal of Physiology·D C Hellam, R J Podolsky
Jan 1, 1980·Annual Review of Physiology·M Bárány, K Bárány
Apr 1, 1981·The Biochemical Journal·J A CoxE A Stein
Jul 1, 1981·Canadian Journal of Biochemistry·W W Bronstein, H R Knull
Jul 1, 1980·Biochimica Et Biophysica Acta·R Y Tsien, T J Rink
Oct 31, 1962·Biochemical and Biophysical Research Communications·D PETTE, H BRANDAU
Sep 24, 1963·Biochimica Et Biophysica Acta·M KASAI, F OOSAWA
Feb 12, 1962·Biochimica Et Biophysica Acta·M KASAIF OOSAWA

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Citations

Jun 1, 1992·Journal of Muscle Research and Cell Motility·J E EstesL C Gershman
Jun 1, 1986·Journal of Muscle Research and Cell Motility·L A SeldenJ E Estes
Jan 1, 1986·Annals of the New York Academy of Sciences·A V SomlyoA P Somlyo
Oct 1, 1987·Journal of Muscle Research and Cell Motility·H G Zot, J D Potter
May 7, 2020·International Journal of Molecular Sciences·Kiyotaka TokurakuTaro Q P Uyeda
Mar 20, 2021·Biochemical and Biophysical Research Communications·Naoki HosokawaKiyotaka Tokuraku
Feb 7, 1997·Journal of Molecular Biology·A Orlova, E H Egelman
Apr 29, 1998·Archives of Biochemistry and Biophysics·I DalleDonneR Colombo
Apr 6, 1989·Biochimica Et Biophysica Acta·L C GershmanJ E Estes
Apr 8, 1991·Biochimica Et Biophysica Acta·H J KinosianL C Gershman
Oct 24, 2006·Biophysical Journal·Marie E Cantino, Abraham Quintanilla
Sep 26, 2003·Biophysical Journal·Dmitry S Kudryashov, Emil Reisler

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