Calcium and spermine interaction with phospholipid bilayers: a 15N NMR study

Biochimie
S Akoka, C Tellier

Abstract

The binding of Ca2+ to membrane models composed of diplamitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylethanolamine (DPPE) 15N-labeled in the polar head group was investigated at pH 8.5 and pH 9.4 by 15N-NMR spectroscopy. Both phospholipids exhibit a decrease in the chemical shift anisotropy, indicating changes of the order parameter of the C-N bond and decrease in half-height width. Binding of Ca2+ induces a chemical shift change for the DPPE signal which indicates a decrease in the pKa of the amino group. The binding of spermine was also investigated for mixed phase (DPPC/DPPE) at pH 8 and pH 9.4; a decrease in the DPPE pKa was also noted. The signals of both phospholipids are broadened and the line shapes are more complex because of the lower mobility and the higher steric bulk of this molecule. The results show the value of 15N-NMR in the study of mixed liposomes and indicate that the deprotonation of membrane surface could constitute a necessary step for fusion processes.

References

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Mar 29, 2013·Proceedings of the National Academy of Sciences of the United States of America·Mylène HugoniPierre E Galand

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Citations

Jan 1, 1992·Archives of Environmental Contamination and Toxicology·A VenturinoA M Pechen de D'Angelo
Apr 1, 1992·Cellular and Molecular Neurobiology·K SzczawinskaV A Eterović
Jan 1, 1991·The International Journal of Biochemistry·T Cserháti, M Szögyi
Jan 1, 1994·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·C M Henley

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