PMID: 2505254Sep 1, 1989Paper

Calcium buffer injections block fucoid egg development by facilitating calcium diffusion

Proceedings of the National Academy of Sciences of the United States of America
J E SpeksnijderL F Jaffe

Abstract

The polarity of fucoid eggs is fixed either when tip growth starts or a bit earlier. A steady flow of calcium ions into the incipient tip is thought to establish a high calcium zone that is needed for its localization and formation. To test this hypothesis, we have injected seven different 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA)-type calcium buffers into Pelvetia eggs many hours before tip growth normally starts. Critical final cell concentrations of each buffer prove to block outgrowth (as well as cell division) for up to 2 weeks. This critical inhibitory concentration is lowest for two buffers with dissociation constants or Kd values of 4-5 x 10(-6) M and increases steadily as the buffers' Kd values shift either below or above this optimal value to ones as low as 4 x 10(-7) M or as high as 9.4 x 10(-5) M. To analyze these results, we have derived an equation (based on the concept of facilitated diffusion) for the effects of diffusable calcium buffers on steady-state calcium gradients. The data fit this equation quite well if it is assumed that cytosolic free calcium at the incipient tip is normally kept at about 7 microM and, thus, far above the general cytosolic level.

References

Jan 10, 1975·Science·K R Robinson, L F Jaffe
Jun 24, 1987·Biochimica Et Biophysica Acta·C H van Os
Dec 1, 1987·Cell Calcium·B S Donahue, R F Abercrombie
Nov 1, 1987·The Journal of Cell Biology·P K Hepler, D A Callaham
Dec 1, 1973·Developmental Biology·K R Robinson, L F Jaffe
Apr 1, 1972·Developmental Biology·R D AllenL F Jaffe
Jan 1, 1984·Progress in Biophysics and Molecular Biology·R Eckert, J E Chad
Sep 17, 1984·Biochemical and Biophysical Research Communications·U QuastV M Doyle
Nov 30, 2011·The Journal of Physiology·Stephen HollingworthStephen M Baylor

❮ Previous
Next ❯

Citations

Aug 10, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·L F Jaffe
Jul 12, 1991·Biochimica Et Biophysica Acta·A A OseiR H Himes
Jun 18, 2003·Biochemical and Biophysical Research Communications·Naihan XuDonald C Chang
Feb 1, 1993·Zygote : the Biology of Gametes and Early Embryos·F Berger, C Brownlee
Jun 25, 1998·Development, Growth & Differentiation·C F LeungA L Miller
Jul 27, 2001·Pathology International·T IshigamiN Maruyama
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·L F Jaffe
Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S Gilroy, R L Jones
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·F A SuprynowiczT Whalley
Dec 1, 1989·The Journal of Cell Biology·P K Hepler
Jul 1, 1990·The Journal of Cell Biology·J P KaoR A Steinhardt
Dec 1, 1991·The Journal of Cell Biology·R A FluckL F Jaffe
Jul 1, 1993·The Journal of Cell Biology·P Snow, R Nuccitelli
Apr 16, 2002·Molecular Biology of the Cell·Tatsuhiko Noguchi, Issei Mabuchi
Mar 12, 2005·BMC Cell Biology·Ralph H SchaloskeChristina Schlatterer
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·U Homann, M Tester
Jul 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·M LéonettiF Homblé
Aug 12, 2008·Journal of Insect Physiology·Anne M Cieniewicz, Richard I Woodruff
Oct 1, 1994·Cell Calcium·P K Hepler
Dec 1, 1991·Bioscience Reports·F M Harold
Jul 1, 1997·Developmental Biology·K R Robinson, B J Miller
Sep 6, 2001·Seminars in Cell & Developmental Biology·C BrownleeF Corellou
Jan 2, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M J Hubbard
Nov 1, 1994·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M TymianskiM C Wallace
Feb 16, 1999·Journal of Neurotrauma·R L MacdonaldB Weir
May 6, 1993·Nature·W M Roberts
Jan 24, 2003·The Journal of Biological Chemistry·Calum LeckieMichael Whitaker
Sep 30, 1999·Microscopy Research and Technique·R CrétonL F Jaffe
Nov 1, 1990·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Danica ZivkovicM René Dohmen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.