Calcium carbonate supplementation to chorioallantoic membranes improves hatchability in shell-less chick embryo culture.

Journal of Bioscience and Bioengineering
Yutaka TaharaMasamichi Kamihira

Abstract

Developing chick embryos are a classical research tool in developmental biology. The whole embryo culture technique can be applied to various fields, such as embryo manipulation, toxicology, tumorigenesis, and basic research in regenerative medicine. When used for the generation of transgenic chickens, a high hatchability of genetically engineered embryos is essential to support normal embryonic development during culture. In this study, calcium carbonate, which is the main component of eggshells, was added as a calcium source in shell-less chick embryo cultures using a transparent plastic film as a culture vessel. In the absence of a calcium source in the shell-less culture system, embryogenesis ceased during culture, resulting in failed embryonic hatching. We found that the direct addition of calcium carbonate to the chorioallantoic membrane of the developing embryo was effective for the hatching of cultured chick embryos. The amount, timing, and location of calcium carbonate addition were investigated to maximize the hatchability of cultured embryos. Starting from the time of calcium carbonate supplementation, >40% hatchability was obtained with the optimal condition. This established method of shell-less chick embryo cultur...Continue Reading

References

May 1, 1971·Poultry Science·J H Elliott, J Bennett
Sep 4, 1998·Development, Growth & Differentiation·M KamihiraS Iijima
Sep 6, 2008·Birth Defects Research. Part C, Embryo Today : Reviews·Cynthia M Coleman
May 12, 2009·Journal of Biotechnology·Masamichi KamihiraShinji Iijima
Aug 5, 2009·Transgenic Research·Carlos Alberto PennoMasamichi Kamihira
Jun 28, 2014·Experimental Cell Research·Domenico Ribatti
Aug 21, 2014·Angiogenesis·Patrycja Nowak-SliwinskaM Luisa Iruela-Arispe
Apr 22, 2018·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Tong WuRong-Rong He
Oct 26, 2018·Methods in Molecular Biology·Ellen J CollariniMarie-Cecile Van de Lavoir

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Related Papers

The Journal of Experimental Zoology. Supplement : Published Under Auspices of the American Society of Zoologists and the Division of Comparative Physiology and Biochemistry
B E DunnK E Scharf
© 2021 Meta ULC. All rights reserved