PMID: 2426390Jul 1, 1986Paper

Calcium channel currents in pars intermedia cells of the rat pituitary gland. Kinetic properties and washout during intracellular dialysis

The Journal of General Physiology
G Cota

Abstract

Ca channel currents in primary cultured pars intermedia cells were studied using whole-cell recording with patch pipettes. Experiments were carried out at 18-21 degrees C in cells internally dialyzed with K-free, EGTA-containing solutions and in the presence of 10 mM Ca or 10 mM Ba in the external solution. Ca and Ba currents depended on the activity of two main populations of channels, SD and FD. With Ca as the charge carrier, these two populations differed in their closing time constants at -80 mV (SD, 1.8 ms; FD, 110 microseconds), apparent activation levels (SD, -40 mV; FD, -5 mV), half-maximal activation levels (SD, +5 to +10 mV; FD, +20 to +25 mV), half-times of activation at +20 mV (SD, 2.5-3.5 ms; FD, 1.0-1.3 ms), and time courses of inactivation (SD, fast; FD, slow). Functional FD channels were almost completely lost within 20-25 min of breaking into a cell, whereas SD channels retained most of their functional activity. In addition, the conductance-voltage curve for FD channels shifted approximately 15 mV toward more negative membrane potentials within 11-14 min under whole-cell recording. At that time, 60-70% of the FD channel maximum conductance was lost. However, the conductance-voltage curve for SD channels shifte...Continue Reading

References

May 1, 1975·The Journal of General Physiology·S HagiwaraO Sand
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·P S Taraskevich, W W Douglas
Nov 1, 1977·The Journal of General Physiology·F Bezanilla, C M Armstrong
Mar 1, 1978·Acta Physiologica Scandinavica·S Ozawa, O Sand
Jan 1, 1985·The Journal of Membrane Biology·I B Levitan
Jan 1, 1986·Biomedica Biochimica Acta·B NiliusR W Tsien
May 1, 1985·The Journal of General Physiology·P Forscher, G S Oxford
Jan 1, 1984·Progress in Biophysics and Molecular Biology·R Eckert, J E Chad
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
May 1, 1982·Peptides·T L O'Donohue, D M Dorsa
Jan 1, 1983·Annual Review of Physiology·R W Tsien
Mar 1, 1984·The Journal of General Physiology·J M Dubinsky, G S Oxford
Mar 1, 1984·The Journal of General Physiology·D R Matteson, C M Armstrong
Sep 1, 1984·Biophysical Journal·E Carbone, H D Lux

Citations

Aug 1, 1992·The Journal of Membrane Biology·T DurrouxM D Payet
Aug 1, 1994·Calcified Tissue International·J LozaS Simasko
Jan 1, 1991·Pflügers Archiv : European journal of physiology·J M Simard
Mar 1, 1992·Pflügers Archiv : European journal of physiology·R Schneggenburger, J López-Barneo
Oct 1, 1993·Pflügers Archiv : European journal of physiology·L Kocmur, Robert Zorec
Jun 1, 1993·Pflügers Archiv : European journal of physiology·K S ElmslieS W Jones
Jun 1, 1988·Pflügers Archiv : European journal of physiology·P G KostyukA N Savchenko
Nov 1, 1987·Pflügers Archiv : European journal of physiology·G Suarez-Kurtz, J P Reuben
Jan 1, 1991·Neir̆ofiziologiia = Neurophysiology·A F Fomina, R E Shirokov
Jan 1, 1990·Journal of Molecular Neuroscience : MN·P G Kostyuk
Dec 13, 1988·Brain Research·E F Stanley, J T Russell
Jan 1, 1989·Neurochemistry International·C ChenJ M Israel
Mar 29, 1996·Neuroscience Letters·L Kocmur-Bobanović, Robert Zorec
Jan 1, 1989·Neuroscience·P G Kostyuk
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C J CohenH Rasmussen
May 1, 1994·The Journal of General Physiology·E A ErtelC J Cohen
Aug 1, 1988·The Journal of General Physiology·D Swandulla, C M Armstrong
Mar 1, 1989·The Journal of General Physiology·L TabaresJ López-Barneo
May 1, 1989·The Journal of General Physiology·J López-LópezJ López-Barneo
May 1, 1989·The Journal of General Physiology·J UreñaJ López-Barneo
Jun 1, 1989·The Journal of General Physiology·A E Lacerda, A M Brown
Feb 1, 1991·The Journal of General Physiology·A Castellano, J López-Barneo
May 31, 1993·Annals of the New York Academy of Sciences·J A ValentijnL Cazin
Dec 1, 1996·Trends in Endocrinology and Metabolism : TEM·Robert Zorec
Aug 1, 1996·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·D M BeattyB M Chronwall
Jan 1, 1989·Annals of the New York Academy of Sciences·S A DeRiemer
Mar 31, 2015·Progress in Neurobiology·Felix NeumaierToni Schneider
Apr 23, 2008·Current Protocols in Neuroscience·M B Jackson
Feb 18, 2018·The Journal of General Physiology·Felix NeumaierToni Schneider
Dec 31, 2002·Physiological Reviews·Edward Perez-Reyes
May 31, 1993·Annals of the New York Academy of Sciences·R SchlichterP Feltz
Jan 1, 1988·The American Journal of Physiology·C Marchetti, A M Brown
Jul 1, 1987·The American Journal of Physiology·J J EnyeartP M Hinkle
Sep 1, 1990·The American Journal of Physiology·B A Biagi, J J Enyeart
Dec 1, 1990·The American Journal of Physiology·G CotaJ L Torres-Escalante
Feb 18, 2019·Cell Calcium·Tomohiko KayanoIzumi Shibuya

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