PMID: 7523660Jun 1, 1994Paper

Calcium channel subtypes in cat chromaffin cells

The Journal of Physiology
A AlbillosE Carbone

Abstract

1. Using the patch-clamp technique we have investigated the kinetic and pharmacological properties of high-voltage-activated (HVA) Ca2+ channels in short-term-cultured cat chromaffin cells. 2. In 10 mM Ba2+, HVA currents activated around -40 mV, reached maximal amplitude at 0 mV and reversed at about +60 mV. At 0 mV, HVA current activation was fast (mean tau act, 2.45 ms), and followed by either an incomplete inactivation or by a second slow phase of activation (mean tau slow, 36.8 ms) that was lost when Ba2+ was replaced by Ca2+. HVA Ba2+ currents deactivate quickly on repolarization to -50 mV (mean tau deact, 0.36 ms). 3. In most cells, HVA currents were sensitive to common dihydropyridine (DHP) derivatives. Nisoldipine blocked the currents maximally at low membrane potentials (mean block 76% at -30 mV, 3 microM) and gradually less at higher voltages. Nisoldipine block was clearly time dependent (33 and 56% after 30 and 600 ms, respectively, to 0 mV). 4. Bay K 8644 (3 microM) action was variable and caused (1) a 2- to 4-fold increase of Ba2+ currents at -40 to -20 mV, (2) a -15 mV shift of the current-voltage relationship and (3) a 10- to 20-fold prolongation of HVA channel deactivation at -50 mV. 5. Nisoldipine block and Bay...Continue Reading

Citations

May 15, 2012·Journal of Molecular Neuroscience : MN·Juliana M RosaLuis Gandía
Jun 1, 1994·Pflügers Archiv : European journal of physiology·M G LópezA R Artalejo
Aug 15, 2000·European Journal of Pharmacology·M F Cano-AbadM G López
Mar 7, 1996·European Journal of Pharmacology·L GandíaR Borges
May 1, 1995·Pflügers Archiv : European journal of physiology·L GandíaA G García
Feb 29, 2000·Journal of Neurochemistry·E García-PalomeroA M Cárdenas
Oct 16, 1999·The Journal of Physiology·T NagayamaS Satoh
Nov 17, 2001·The Journal of Physiology·R BournaudT Shimahara
Aug 24, 1999·The European Journal of Neuroscience·E A Lukyanetz, E Neher
Nov 21, 2007·Acta Physiologica·A P FoxZ Xie
Oct 1, 1996·Journal of Autonomic Pharmacology·P M Lundy, R Frew
Sep 1, 1995·British Journal of Pharmacology·S J Hong, C C Chang
Dec 19, 2016·European Journal of Pharmacology·Alicia Hernández-VivancoAlmudena Albillos
Apr 1, 1995·Pflügers Archiv : European journal of physiology·V MagnelliE Carbone
Mar 20, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Tsuyoshi AkiyamaKenji Sunagawa
Sep 4, 2017·Pflügers Archiv : European journal of physiology·Carmen NanclaresLuis Gandía
Apr 30, 2008·Pflügers Archiv : European journal of physiology·Alberto Pérez-AlvarezAlmudena Albillos
Nov 20, 2002·Annals of the New York Academy of Sciences·Inmaculada Cuchillo-IbanezAntonio G Garcia
Jan 1, 1996·Pflügers Archiv : European journal of physiology·K OtsuguroY Nakazato
Oct 4, 2006·Physiological Reviews·Antonio G GarcíaJavier García-Sancho
Dec 17, 2010·American Journal of Physiology. Cell Physiology·A HernándezA Hernández-Cruz
Aug 5, 2017·Pflügers Archiv : European journal of physiology·Christopher J LingleEmilio Carbone

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