Calcium channels in chicken sperm regulate motility and the acrosome reaction

The FEBS Journal
Thi Mong Diep NguyenElisabeth Blesbois

Abstract

Intracellular cytoplasmic calcium ([Ca(2+) ]i ) has an important regulatory role in gamete functions. However, the biochemical components involved in Ca(2+) transport are still unknown in birds, an animal class that has lost functional sperm-specific CatSper channels. Here, we provide evidence for the presence and expression of various Ca(2+) channels in chicken sperm, including high voltage-activated channels (L and R types), the store-operated Ca(2+) channel (SOC) component Orai1, the transient receptor potential channel (TRPC1) and inositol-1,4,5-trisphosphate receptors (IP3 R1). L- and R-type channels were mainly localized in the acrosome and the midpiece, and T-type channels were not detected in chicken sperm. Orai1 was found in all compartments, but with a weak, diffuse signal in the flagellum. TRCP1 was mainly localized in the acrosome and the midpiece, but a weak diffuse signal was also observed in the nucleus and the flagellum. IP3 R1 was mainly detected in the nucleus. The L-type channel inhibitor nifedipine, the R-type channel inhibitor SNX-482 and the SOC inhibitors MRS-1845, 2-APB and YM-58483 decreased [Ca(2+) ]i sperm motility and acrosome reaction capability, with the SOC inhibitors inhibiting these functions mo...Continue Reading

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Citations

Nov 15, 2017·Journal of Cellular Physiology·Julio C ChávezAlberto Darszon
Oct 27, 2018·International Journal of Molecular Sciences·David Martin-HidalgoM Julia Bragado
Jan 21, 2021·Animals : an Open Access Journal From MDPI·María Gemma Millán de la BlancaJulián Santiago-Moreno
Dec 6, 2020·International Journal of Molecular Sciences·Xiaoning ZhangXuhui Zeng
Jul 28, 2020·Molecular and Cellular Endocrinology·Maya FinkelsteinHaim Breitbart
Apr 4, 2021·International Journal of Molecular Sciences·Karolina Nowicka-Bauer, Monika Szymczak-Cendlak

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