Calcium channels in neuroblastoma cell growth cones

The European Journal of Neuroscience
F Zimprich, S Bolsover

Abstract

The concentration of free calcium ions in the cytosol has been shown to influence many components of growth cone behaviour, including the extension of filopodia and veils, the addition of new membrane to the plasmalemma, the retraction and disappearance of filopodia, and gross collapse and retraction of the growth cone. A spatially localized modulation of these processes by very local calcium changes has been proposed to underlie the steering of growth cones by gradients of neurotransmitters, voltage and cell adhesion molecules. Such local control can be studied in mouse neuroblastoma cells, where depolarization causes calcium to rise in a limited number of spatially restricted hotspots, triggering a localized advance. We have studied the simple, club-shaped growth cones that are characteristically found on advancing neurites. Depolarization caused calcium to increase most at the distal, leading tip. Agents that disrupt calcium-induced calcium release do not affect growth cone calcium dynamics, ruling out a local release of calcium at the tip as a cause of the gradient. Using cell-attached patch recording, we find that L-type calcium channels are present at a higher density at the distal tip than in the proximal growth cone. Ou...Continue Reading

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Citations

Aug 17, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·N C SpitzerT M Gomez
May 2, 2012·Proceedings of the National Academy of Sciences of the United States of America·J Stefan KaczmarekDavid E Clapham
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