Calcium compartments in brain

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
George C NewmanKeith W Jones

Abstract

Excellent progress has been made toward understanding the physiology and pharmacology of specific calcium-related cellular processes of the brain, but few studies have provided an integrated view of brain calcium kinetics. To further the knowledge of the size and binding properties of brain calcium compartments, the authors have conducted a series of experiments in hippocampal brain slices exposed to high and low extracellular calcium. Slices were incubated in buffers containing 0.001 to 4.5 mmol/L calcium for up to 75 minutes. Slice calcium content was analyzed by three methods: exchange equilibrium with 45Ca, synchrotron-radiation-induced x-ray emission, and inductively coupled plasma. Data were analyzed using a model based on a Langmuir isotherm for two independent sites, with additional extracellular and bound compartments. In parallel experiments, altered low calcium had no effect on slice histology and only mild effects on slice adenylates. When combined with prior 45Ca and fluorescent probe binding experiments, these results suggest that there are at least five kinetically distinct calcium compartments: (1) free extracellular (approximately 10%); (2) loosely associated extracellular plasma membrane (approximately 55%); (...Continue Reading

References

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Citations

Aug 9, 2008·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Linda K FriedmanRobert Keesey
Oct 22, 2020·Metallomics : Integrated Biometal Science·Brandon MahanFrédéric Moynier
Sep 15, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Ushodaya MattamNaresh Babu V Sepuri

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Methods Mentioned

BETA
x-ray microprobe

Software Mentioned

SPSS
Curvefit
PC
True Basic
SRIXE

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