PMID: 7019912May 1, 1981Paper

Calcium control of microfilaments: uncoupling of the F-actin-severing and -bundling activity of villin by limited proteolysis in vitro

Proceedings of the National Academy of Sciences of the United States of America
J R Glenney, K Weber

Abstract

Villin is a major F-actin-bundling protein present in the microfilament bundle underlying the plasma membrane of the microvilli present on intestinal epithelial cells. Mild in vitro proteolysis converts villin (Mr, 95,000) into a large fragment, the villin core (apparent Mr, 90,000). Villin core has lost the F-actin-bundling activity expressed by villin in the absence of calcium but retains the micromolar Kd calcium-binding site and the calcium-dependent restriction of actin filament length (F-actin severing) of intact villin. This finding suggests a common structural and functional relatedness between the known calcium-dependent F-actin-severing proteins from different cell types, even though not all of them reveal F-actin-bundling activity.

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Citations

Dec 11, 1997·The Journal of Experimental Zoology·H F Cantiello
Sep 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·E FriederichD Louvard
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