PMID: 7546746Sep 1, 1995Paper

Calcium control of transmitter release at a cerebellar synapse

Neuron
I M MintzW G Regehr

Abstract

The manner in which presynaptic Ca2+ influx controls the release of neurotransmitter was investigated at the granule cell to Purkinje cell synapse in rat cerebellar slices. Excitatory postsynaptic currents were measured using whole-cell voltage clamp, and changes in presynaptic Ca2+ influx were determined with the Ca(2+)-sensitive dye furaptra. We manipulated presynaptic Ca2+ entry by altering external Ca2+ levels and by blocking Ca2+ channels with Cd2+ or with the toxins omega-conotoxin GVIA and omega-Aga-IVA. For all of the manipulations, other than the application of omega-Aga-IVA, the relationship between Ca2+ influx and release was well approximated by a power law, n approximately 2.5. When omega-Aga-IVA was applied, release appeared to be more steeply dependent on Ca2+ (n approximately 4), suggesting that omega-Aga-IVA-sensitive channels are more effective at triggering release. Based on interactive effects of toxins on synaptic currents, we conclude that multiple types of Ca2+ channels synergistically control individual release sites.

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Citations

Jun 8, 2006·Pflügers Archiv : European journal of physiology·E CarboneV Carabelli
Mar 6, 2010·Pflügers Archiv : European journal of physiology·Daniela Pietrobon
Sep 1, 2006·Cell and Tissue Research·Tobias MoserAnna Lysakowski
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