Calcium currents in human and canine jejunal circular smooth muscle cells

Gastroenterology
G FarrugiaJ H Szurszewski

Abstract

Although calcium plays an essential role in intestinal smooth muscle contractile activity, calcium entry pathways in canine and human small intestine are largely unknown. The goal of this study was to characterize calcium channels, a potential entry pathway for calcium, in isolated circular smooth muscle cells of canine and human jejunum. Single freshly dissociated human and canine jejunal circular smooth muscle cells were studied using single-channel and perforated whole-cell patch clamp recordings as well as fluorescence dual wavelength ratio imaging. An inward whole-cell current was identified that was carried by a 17 pS (80 mmol/L Ba2+) dihydropyridine-sensitive, barium-permeable channel. The current was potentiated by BayK 8644 (1 mumol/L; n = 3; 82% +/- 34%), acetylcholine (1 mumol/L; n = 8; 42% +/- 5%), and erythromycin (1 mumol/L; n = 9; 70% +/- 11%) and was completely blocked by nifedipine (1 mumol/L; n = 6) or diltiazem (200 mumol/L; n = 4). Application of BayK 8644 (1 mumol/L), acetylcholine (1 mumol/L), or erythromycin (1 mumol/L) to Fura-2-loaded smooth muscle cells bathed in Krebs' solution containing 2.54 mmol/L calcium increased intracellular calcium levels. A calcium entry pathway was identified in canine and h...Continue Reading

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Citations

May 9, 2007·Annals of Biomedical Engineering·Alberto Corrias, Martin L Buist
Sep 5, 2003·European Journal of Pharmacology·Luke J Janssen, Jennifer Wattie
Apr 1, 1999·Annual Review of Physiology·B HorowitzK M Sanders
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Mar 12, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Peter R StregeGianrico Farrugia
Jan 31, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Robert E KraichelyGianrico Farrugia
Aug 23, 2012·PloS One·Yong Cheng PohMartin Lindsay Buist
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Jun 17, 2004·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·A MuinuddinN E Diamant
Mar 30, 2007·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·R E Kraichely, G Farrugia
Apr 18, 2008·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·K M Sanders
Jan 25, 2007·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·P R StregeG Farrugia
Jun 5, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ahmad MuinuddinNicholas E Diamant
Jul 17, 1999·The American Journal of Physiology·A EppersonB Horowitz
Feb 9, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·X Z Shi, S K Sarna

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