Calcium-dependence of hydrogen peroxide-induced c-fos expression and growth stimulation of multicellular prostate tumor spheroids

FEBS Letters
H SauerM Wartenberg

Abstract

Hydrogen peroxide (H2O2) in nanomolar concentrations (20-100 nM) stimulated the growth of small (diameter 100 +/- 30 microm) multicellular prostate cancer spheroids and increased c-fos expression. H2O2 transiently raised [Ca2+]i by Ca2+ release from intracellular stores as the transient persisted in low (10 nM) Ca2+ solution but was abolished when intracellular Ca2+ stores were depleted by thapsigargin or chelation of [Ca2+]i with BAPTA. The H2O2-induced [Ca2+]i transient was furthermore inhibited by the P2-purinoreceptor antagonists suramin and basilen blue, indicating that H2O2 may act via purinergic receptor stimulation. Treatment of spheroids with either suramin, basilen blue or BAPTA inhibited the H2O2-induced growth stimulation and c-fos expression, indicating that the H2O2-mediated growth stimulation of multicellular spheroids is mediated via a Ca2+-dependent pathway.

References

Sep 22, 1992·Biochemistry·C R MiddaughK E Marfia
Jan 1, 1989·Free Radical Research Communications·R H Burdon, C Rice-Evans
Jan 25, 1985·Science·P A Cerutti
Jun 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M J Berridge
May 1, 1995·The European Journal of Neuroscience·J D RobackB H Wainer
Jan 1, 1994·Annual Review of Immunology·P A Baeuerle, T Henkel
Dec 22, 1995·The Journal of Biological Chemistry·V J Thannickal, B L Fanburg
Feb 1, 1996·Journal of Cellular Physiology·K TörnquistB Dugué
Feb 23, 1996·The Journal of Biological Chemistry·K Z GuytonN J Holbrook
Jan 1, 1995·Micron : the International Research and Review Journal for Microscopy·M Wartenberg, H Acker
Jan 1, 1996·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·B LiuR L Whisler
Jun 28, 1996·The Journal of Biological Chemistry·Y Y LoT F Cruz
Sep 1, 1996·The Biochemical Journal·M SundaresanT Finkel
Jan 1, 1997·Free Radical Biology & Medicine·Y J SuzukiA Sevanian
Mar 14, 1997·Science·K IraniP J Goldschmidt-Clermont

❮ Previous
Next ❯

Citations

Jul 19, 2001·Biotechnology and Bioengineering·R M EnmonR S Dotson
Dec 23, 2011·Breast Cancer Research and Treatment·Emman ShubbarCharlotta Enerbäck
Jan 21, 2000·Free Radical Biology & Medicine·H SauerM Wartenberg
Oct 13, 2000·British Journal of Pharmacology·G NickenigM Böhm
Nov 1, 2003·Antioxidants & Redox Signaling·Koichi NiwaMikinori Kuwabara
May 19, 2006·Photomedicine and Laser Surgery·Rachel LubartShimon Rochkind
Sep 24, 1999·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·G SersaD J Chaplin
Sep 14, 2010·In Vitro Cellular & Developmental Biology. Animal·J Antonio Heredia-RojasReyes S Taméz-Guerra
Apr 15, 2005·Redox Report : Communications in Free Radical Research·Yi Zheng, Xun Shen
Jun 3, 2004·Chembiochem : a European Journal of Chemical Biology·Hidenori OtsukaKazunori Kataoka
Sep 25, 2004·The Prostate·So Dug LimRebecca S Arnold
Oct 20, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·H SauerM Wartenberg
Sep 20, 2020·International Journal of Molecular Sciences·Fabrizio FontanaPatrizia Limonta

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.