PMID: 6405758Mar 1, 1983Paper

Calcium dependency of aortic smooth muscle cell migration induced by 12-L-hydroxy-5,8,10,14-eicosatetraenoic acid. Effects of A23187, nicardipine and trifluoperazine

Atherosclerosis
J NakaoS Murota

Abstract

We have previously reported that 12-L-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE), a 12-lipoxygenase product of arachidonic acid in platelets, is a potent chemoattractant for rat aortic smooth muscle cells. In the present study, the mechanism involved in 12-HETE-associated smooth muscle cell migration was investigated in relation to calcium mobilization in the cells. Migration of smooth muscle cells was measured by a filter membrane technique in modified Boyden chambers. Smooth muscle cell migration induced by 12-HETE increased with the increase of extracellular Ca2+ concentration and became maximal at the physiological Ca2+ concentration of 1.25 mM. The calcium ionophore A23187, at concentrations of 0.2 and 2.0 microM, significantly stimulated cell migration. Nicardipine, a potent calcium-entry blocker, significantly inhibited 12-HETE-associated smooth muscle cell migration at concentrations from 10(-9) to 10(-5) M. Concentrations of trifluoperazine from 10(-9) to 10(-5) M and W-7 at 10(-5) M, which are specific inhibitors of calmodulin, also significantly inhibited cell migration induced by 12-HETE. Cytochalasin B at 1.0 and 10 microM, and colchicine at 0.1 and 1.0 microM concentrations drastically inhibited cell migrat...Continue Reading

References

May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P Borgeat, B Samuelsson
Feb 6, 1970·Biochemical and Biophysical Research Communications·W Y Cheung
Jan 1, 1970·Advances in Biochemical Psychopharmacology·W Y Cheung
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L OrningB Samuelsson
May 1, 1981·The Journal of Clinical Investigation·P H NaccacheE J Goetzl
Mar 15, 1982·The Biochemical Journal·W C ChangS Murota
Jun 1, 1980·Cell·S MacLean-Fletcher, T D Pollard
Mar 1, 1981·The Journal of Cell Biology·S S Brown, J A Spudich
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G R GrotendorstG R Martin
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H HidakaT Nagata
Feb 15, 1981·Biochemical Pharmacology·M TeraiH Maeno

❮ Previous
Next ❯

Citations

Jan 1, 1993·World Journal of Surgery·O GökçeY Güngen
Aug 1, 1990·Cardiovascular Drugs and Therapy·P D Henry
Aug 1, 1990·Cardiovascular Drugs and Therapy·A M Knorr, S Kazda
Aug 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·O Tokunaga, T Watanabe
Apr 25, 1991·European Journal of Pharmacology·G Sperti, W S Colucci
Mar 6, 1998·International Journal of Cardiology·M Schachter
Sep 25, 2008·Nature Clinical Practice. Nephrology·Charles J DiskinThomas B Carter
Mar 4, 2000·The Australian and New Zealand Journal of Surgery·P Y AoJ P Fletcher
May 1, 1992·European Journal of Epidemiology·S JostP Lichtlen
Nov 5, 2005·Molecular Nutrition & Food Research·Hartmut KühnJutta Belkner
Sep 16, 1985·Biochemical and Biophysical Research Communications·R Shankar, J D Sallis
Dec 18, 1990·The American Journal of Cardiology·C V Ram
Dec 1, 1994·American Heart Journal·D Waters, J Lespérance
Jan 9, 1992·Biochemical Pharmacology·W G Nayler
Oct 6, 2007·Expert Opinion on Drug Metabolism & Toxicology·Kwo-Chang UengChung-Sheng Lin
Jan 1, 1989·Clinical and Experimental Hypertension. Part A, Theory and Practice·W KiowskiF R Bühler
Oct 24, 2007·Journal of Pharmacological Sciences·Yong-Ri JinYeo-Pyo Yun
Mar 24, 2007·Biochemical and Biophysical Research Communications·Gary WeisingerNaftali Stern
Jun 29, 1999·The Journal of Pharmacy and Pharmacology·Z KerryA Ozer
Jun 1, 1985·Journal of Autonomic Pharmacology·G Feuerstein
Jan 1, 1988·Annals of the New York Academy of Sciences·P D Henry
Jun 1, 1991·American Heart Journal·R SahniV S Banka
Sep 1, 1985·Circulation·P D Henry

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.