PMID: 8947482Nov 15, 1996Paper

Calcium-dependent activation of Erk-1 and Erk-2 after hypo-osmotic astrocyte swelling

The Biochemical Journal
F SchliessD Häussinger

Abstract

The influence of hypo-osmotic cell swelling on the activity of the mitogen-activated protein (MAP) kinases Erk-1 and Erk-2 (where Erk stands for extracellular signal-regulated protein kinase) was studied in cultured rat astrocytes. Hypo-osmotic treatment led within 10 min to an increased activity of Erk-1 and Erk-2, which became maximal at 20 min and returned to the basal level within 60 min. Moreover, exposure to hypo-osmotic conditions induced a biphasic increase in cytosolic Ca2+ concentration ([Ca2+]i): a rapid peak-like increase was followed by a sustained plateau. The absence of extracellular Ca2+ completely abolished Erk activation as well as the plateau of the [Ca2+]i response after hypo-osmotic stimulation. Application of wortmannin and agents to elevate intracellular cAMP levels also completely blocked Erk activation but were without effect on the biphasic [Ca2+]i response to hypo-osmotic treatment of the cells, suggesting a role of PtdIns 3-kinase and the Ras/Raf pathway downstream of the calcium signal. Protein kinase C (PKC) and Ca2+/calmodulin (CaM)-dependent kinases are unlikely to play a role in the hypo-osmolarity-induced signalling towards MAP kinases, as revealed by the blockage of PKC and CaM kinases. Inhibi...Continue Reading

Citations

May 16, 1998·The Journal of Cell Biology·A Lepple-WienhuesF Lang
Mar 30, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Freimut SchliessDieter Häussinger
Jan 6, 2004·Fundamental & Clinical Pharmacology·Alexandra d'Anglemont de TassignyAlain Berdeaux
Aug 19, 2008·Glia·Caroline KruczekDieter Häussinger
Apr 10, 2010·Biology of the Cell·Audrey FouchsKarine Pichavant-Rafini
Feb 8, 2018·Physiological Reviews·Natalia PrevarskayaYaroslav Shuba
Dec 11, 2002·Journal of Gastroenterology and Hepatology·Dieter HäussingerGerald Kircheis
Jan 24, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Boris GörgFreimut Schliess
Sep 24, 2004·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Dieter Häussinger
Feb 11, 1998·Physiological Reviews·F LangD Häussinger
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·B MurrayE J Furshpan
May 18, 1999·The American Journal of Physiology·A A MonginH K Kimelberg
Jan 31, 2002·World Journal of Gastroenterology : WJG·S vom DahlD Häussinger
Jun 26, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Grazia Paola NicchiaMaria Svelto
Jun 3, 2011·Headache·Saurabh GuptaNancy E J Berman
Dec 2, 2010·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Liping CaiLiang Peng
Feb 8, 2000·American Journal of Physiology. Renal Physiology·Z ZhangD M Cohen
Dec 31, 2005·Metabolic Brain Disease·Dieter HäussingerFreimut Schliess
Sep 17, 2004·American Journal of Physiology. Cell Physiology·Alexander A Mongin, Harold K Kimelberg
Jan 18, 2019·International Journal of Molecular Sciences·Audrey D Lafrenaye, J Marc Simard
Jun 1, 2006·Acta Physiologica·H Pasantes-MoralesG Ramos-Mandujano
May 29, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Boris GörgDieter Häussinger
Nov 4, 2006·Biological Chemistry·Freimut SchliessDieter Häussinger
Jul 15, 1999·Biochemical and Biophysical Research Communications·P W SchenkB E Snaar-Jagalska
Mar 2, 1999·Biochemical and Biophysical Research Communications·D Häussinger, F Schliess
Jun 27, 1998·Biochemical and Biophysical Research Communications·M IkedaB E Sumpio
Jan 4, 2001·Neurochemistry International·S Morales-MuliaH Pasantes-Morales
Apr 10, 2007·Experimental Eye Research·José E Capó-ApontePeter S Reinach

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes & Neurodegeneration

Astrocytes are important for the health and function of the central nervous system. When these cells stop functioning properly, either through gain of function or loss of homeostatic controls, neurodegenerative diseases can occur. Here is the latest research on astrocytes and neurodegeneration.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.