PMID: 11319767Apr 25, 2001Paper

Calcium-dependent association of annexin VI, protein kinase C alpha, and neurocalcin alpha on the raft fraction derived from the synaptic plasma membrane of rat brain

Journal of Neuroscience Research
A OritoS Maekawa

Abstract

A membrane microdomain enriched in cholesterol and sphingolipids or so called "raft" region was found to contain many signal transducing proteins such as GPI-anchored proteins, trimeric G proteins and protein tyrosine kinases. Because brain-derived raft contains two calmodulin-binding proteins, GAP-43 and NAP-22 as the major protein components, the raft domain is assumed to be important in the Ca(2+)-signaling. In this study, we analyzed protein components showing Ca(2+)-dependent binding to the raft of synaptic plasma membrane from rat brain. SDS-PAGE analysis of the protein components in the EGTA eluate from the raft prepared in the presence of Ca(2+)-ions showed the elution of 80 kDa, 68 kDa, 22 kDa, and 21 kDa proteins. These proteins were identified as protein kinase C alpha (80 kDa) and annexin VI (68 kDa) from the partial amino-acid sequencing, and neurocalcin alpha (22 kDa) and calmodulin (21 kDa) with western blotting and electrophoretic mobilities in the presence or absence of Ca(2+) ions. Further immunoblotting experiments showed the Ca(2+)-dependent association of conventional, but not non-conventional, subtypes of PKC to the raft.

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Citations

Sep 25, 2002·Journal of Neuroscience Research·Akira TerashitaShohei Maekawa
Feb 23, 2012·Experimental Brain Research·W Volknandt, M Karas
Feb 9, 2016·Immunology and Cell Biology·Rhea CornelyKatharina Gaus
Jan 11, 2011·Biochemical and Biophysical Research Communications·Magdalena M DomonSlawomir Pikula
Feb 19, 2014·Molecular and Cellular Neurosciences·Jens WeingartenWalter Volknandt
Apr 14, 2004·The Journal of Biological Chemistry·Danielle te VruchteDan J Sillence
Nov 23, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Thomas Grewal, Carlos Enrich
Apr 22, 2009·Cellular and Molecular Life Sciences : CMLS·Katia MonastyrskayaAnnette Draeger
Jun 3, 2005·Journal of Neuroscience Research·Katsutoshi TaguchiShohei Maekawa
Jun 21, 2006·The Journal of Biological Chemistry·Viktoria KheifetsDaria Mochly-Rosen
Mar 28, 2002·Physiological Reviews·Volker Gerke, Stephen E Moss
Oct 3, 2002·The Journal of Biological Chemistry·Tong LiuMan Sun Sy
Oct 15, 2019·Journal of Neurochemistry·Simon Wheeler, Dan J Sillence
Sep 11, 2008·Cell Biology International·Salma LocateJohn H Walker
May 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Carlos LeonNeil E Reiner
Jun 19, 2002·The Journal of Biological Chemistry·Iñaki de DiegoThomas Grewal
Apr 23, 2009·Cellular Signalling·Thomas Grewal, Carlos Enrich
Oct 5, 2010·Biochimica Et Biophysica Acta·Carlos EnrichThomas Grewal

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