Calcium-dependent cyto- and genotoxicity of nickel metal and nickel oxide nanoparticles in human lung cells

Particle and Fibre Toxicology
Sebastiano Di BucchianicoH L Karlsson

Abstract

Genotoxicity is an important toxicological endpoint due to the link to diseases such as cancer. Therefore, an increased understanding regarding genotoxicity and underlying mechanisms is needed for assessing the risk with exposure to nanoparticles (NPs). The aim of this study was to perform an in-depth investigation regarding the genotoxicity of well-characterized Ni and NiO NPs in human bronchial epithelial BEAS-2B cells and to discern possible mechanisms. Comparisons were made with NiCl2 in order to elucidate effects of ionic Ni. BEAS-2B cells were exposed to Ni and NiO NPs, as well as NiCl2, and uptake and cellular dose were investigated by transmission electron microscopy (TEM) and inductively coupled plasma mass spectrometry (ICP-MS). The NPs were characterized in terms of surface composition (X-ray photoelectron spectroscopy), agglomeration (photon cross correlation spectroscopy) and nickel release in cell medium (ICP-MS). Cell death (necrosis/apoptosis) was investigated by Annexin V-FITC/PI staining and genotoxicity by cytokinesis-block micronucleus (cytome) assay (OECD 487), chromosomal aberration (OECD 473) and comet assay. The involvement of intracellular reactive oxygen species (ROS) and calcium was explored using the...Continue Reading

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Citations

Jul 20, 2019·Nanotoxicology·Emma ÅkerlundHanna L Karlsson
Feb 16, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Govind GuptaBengt Fadeel
Sep 25, 2019·International Journal of Molecular Sciences·Hongrui GuoLing Zhao
Oct 18, 2019·Frontiers in Cardiovascular Medicine·Nadhim Kamil HanteMaria Jose Santos-Martinez
Sep 28, 2020·Environmental Science and Pollution Research International·Michal ŠtefančíkMonika Pávková Goldbergová
Nov 30, 2019·Endocrine, Metabolic & Immune Disorders Drug Targets·Thea MagroneEmilio Jirillo
Jan 8, 2021·Biological Trace Element Research·Kumsal KocadalSahan Saygi
Nov 17, 2020·EFSA Journal·UNKNOWN EFSA Panel on Contaminants in the Food Chain (CONTAM)Elsa Nielsen
Feb 12, 2021·Metallomics : Integrated Biometal Science·Ruedeemars YubolphanPornpun Vivithanaporn
Feb 21, 2021·Journal of Inorganic Biochemistry·Lukas MasarykPavel Kopel
Sep 4, 2021·International Journal of Nanomedicine·Sarah McCarrickHanna L Karlsson

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Methods Mentioned

BETA
transmission electron microscopy
X-ray
light-scattering
chromosomal aberration assay
light scattering
cross-correlation spectroscopy
chromosomal aberrations

Software Mentioned

BD Accuri C6
BD
GraphPad Prism

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