Calcium-dependent electrophysiological alterations in hypertrophied rat cardiomyocytes

Biochemical and Biophysical Research Communications
C RuoccoA Mugelli

Abstract

We compared the occurrence of calcium-dependent electrophysiological alterations in left ventricular myocytes isolated from the heart of old spontaneously hypertensive (SHR) and age-matched normotensive Wistar Kyoto rats (WKY). Electrical and mechanical activity were measured in patch-clamped myocytes in which intracellular Ca2+ was allowed to change freely. In parallel experiments, intracellular Ca2+ concentration was monitored in fura-2-AM preloaded cells using an image analysis system. Left ventricular myocytes from SHR showed a significantly greater membrane capacitance (an index of cell size), a prolonged duration of the action potential and a higher incidence of delayed afterdepolarization (DADs) and associated aftercontractions, and of spontaneous activity. DADs were likely generated by intracellular Ca2+ waves due to spontaneous and local Ca2+ release from intracellular stores. These results demonstrate that DADs, possibly related to spontaneous intracellular Ca2+ waves, occur frequently in hypertrophied rat cardiomyocytes and may be a relevant arrhythmogenic mechanism.

Citations

Jun 15, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Martin FinkDenis Noble
Mar 27, 2007·Biochimica Et Biophysica Acta·Romain Guinamard, Patrick Bois
Sep 12, 2001·American Journal of Physiology. Endocrinology and Metabolism·J J LuikenJ F Glatz

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