Calcium-dependent inactivation of high-threshold calcium currents in human dentate gyrus granule cells

The Journal of Physiology
U V Nagerl, I Mody

Abstract

1. Dentate gyrus granule cells acutely dissociated from hippocampal slices obtained from chronic temporal lobe epilepsy (TLE) patients displayed a high-voltage activated (HVA) Ca2+ conductance with a pronounced Ca2+-dependent inactivation. 2. Inactivation time constants and peak HVA Ca2+ current (ICa) amplitudes did not differ between perforated patch and whole-cell recordings without added exogenous Ca2+ buffers, indicating that the Ca2+-dependent characteristics of ICa inactivation were well preserved in whole-cell recordings. 3. Inactivation time constants correlated with whole-cell ICa, and were increased when Ca2+ was replaced with Ba2+ in the external solution or 5 mM BAPTA was added to the pipette solution. 4. In recordings without added exogenous Ca2+ buffers, the time course of ICa inactivation was comparable between human TLE and kindled rat granule cells. Conversely, the time course of ICa in human TLE granule cells loaded with 5 mM intracellular BAPTA resembled that observed in buffer-free recordings from control rat neurones. 5. The loss of a putative intraneuronal Ca2+ buffer, the Ca2+-binding protein calbindin (CB), from human granule cells during TLE may result in the pronounced Ca2+-dependent ICa inactivation. ...Continue Reading

References

Nov 1, 1977·The Journal of General Physiology·F Bezanilla, C M Armstrong
Jan 1, 1991·Progress in Neurobiology·R J Miller
Mar 1, 1991·Journal of Neuroscience Methods·J RaeM Watsky
Nov 1, 1989·Trends in Neurosciences·M B Kennedy
Jan 1, 1989·Comparative Biochemistry and Physiology. A, Comparative Physiology·J Chad
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·P S ChardG D Ghadge
Aug 1, 1994·Journal of Neurophysiology·L S Eliot, D Johnston
Dec 1, 1993·The Journal of Physiology·P S ChardR J Miller
May 1, 1993·Journal of Neurophysiology·R J SayerW E Crill
May 28, 1993·Annals of the New York Academy of Sciences·S J GibbonsR J Miller
Jan 1, 1997·The Journal of Physiology·H BeckC E Elger
Feb 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·M S AiraksinenM Meyer

❮ Previous
Next ❯

Citations

Feb 6, 2004·Progress in Neurobiology·Rafael Gutiérrez
Aug 2, 2002·European Journal of Pharmacology·Owen T Jones
Oct 15, 2010·Cold Spring Harbor Perspectives in Biology·Beat Schwaller
Nov 21, 2009·Journal of Biological Rhythms·Joseph LeSauterRae Silver
Apr 4, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·A Ashleigh LongKendal Broadie
Apr 2, 2002·Experimental Neurology·L Van Den BoschM W Berchtold
Oct 16, 1999·The Journal of Physiology·J PalecekB U Keller
Apr 15, 2011·The Journal of Comparative Neurology·M Angeles RealSalvador Guirado
Feb 6, 2013·Biochimica Et Biophysica Acta·David J Adams, Géza Berecki
Nov 5, 2002·Nature Reviews. Neuroscience·Thomas BuddeHans-Christian Pape
May 10, 2019·International Journal of Molecular Sciences·Tamás Kovács-ÖllerBéla Völgyi
Jul 17, 2019·Cold Spring Harbor Perspectives in Biology·Beat Schwaller
Nov 10, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ju Hee KimHyeon Son

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.