Calcium-dependent inhibition of in vitro thin-filament motility by native titin

FEBS Letters
M S Kellermayer, H Granzier

Abstract

Titin ( also known as connectin) is a giant filamentous protein that spans the distance between the Z- and M-lines of the vertebrate muscle sarcomere and plays a fundamental role in the generation of passive tension. Titin has been shown to bind strongly to myosin, making it tightly associated to the thick filament in the sarcomere. Recent observations have suggested the possibility that titin also interacts with actin, implying further functions of titin in muscle contraction. We show -- using in vitro motility and binding assays -- that native titin interacts with both filamentous actin and reconstituted thin filaments. The interaction results in the inhibition of the filaments' in vitro motility. Furthermore, the titin-thin filament interaction occurs in a calcium-dependent manner: increased calcium results in enhanced binding of thin filaments to titin and greater suppression of in vitro motility.

References

Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·K WangR Ramirez-Mitchell
Feb 1, 1991·Current Opinion in Cell Biology·J Trinick
Jan 1, 1991·Methods in Enzymology·S J KronJ A Spudich
Feb 1, 1989·Journal of Biochemistry·T MurayamaK Maruyama
Sep 11, 1986·Nature·R HorowitsR J Podolsky
Jan 1, 1982·Methods in Enzymology·S S Margossian, S Lowey
Jan 1, 1982·Methods in Enzymology·J M Murray
Jan 1, 1982·Methods in Enzymology·J D Pardee, J A Spudich
May 1, 1994·Biophysical Chemistry·K Maruyama
Sep 1, 1994·The Journal of Cell Biology·K J EilertsenT C Keller
Dec 1, 1993·Journal of Muscle Research and Cell Motility·P VibertB W Elliott
Aug 1, 1993·Journal of Muscle Research and Cell Motility·K Trombitás, G H Pollack
Apr 26, 2005·Biophysical Journal·A NagyM S Z Kellermayer

❮ Previous
Next ❯

Citations

May 23, 2002·Biophysical Journal·Maria A BagniFrancesco Colomo
Jul 25, 2003·Insect Biochemistry and Molecular Biology·Z A PodlubnayaG Beinbrech
Feb 2, 2012·Exercise and Sport Sciences Reviews·Walter HerzogTim R Leonard
Sep 9, 2011·Proceedings. Biological Sciences·Kiisa C NishikawaStan L Lindstedt
Nov 25, 2004·European Journal of Biochemistry·Nicolas NiederländerPatrick Chaussepied
Dec 14, 2011·Journal of Biomedicine & Biotechnology·Charles S ChungHenk L Granzier
May 19, 2010·Circulation·Martin M LeWinter, Henk Granzier
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·L GramaM S Kellermayer
Apr 24, 2010·Journal of Biomedicine & Biotechnology·Hideto FukushimaHenk Granzier
Mar 5, 2016·Biomechanics and Modeling in Mechanobiology·Thomas HeidlaufOliver Röhrle
Jun 18, 2002·Journal of Structural Biology·Wolfgang A LinkeJulio M Fernandez
Jan 1, 1997·Critical Reviews in Biochemistry and Molecular Biology·A Oplatka
May 1, 2012·Journal of Biomechanics·Geoffrey A PowerAnthony A Vandervoort
Feb 13, 2001·Biophysical Journal·M S KellermayerH L Granzier
Nov 26, 2014·Journal of Muscle Research and Cell Motility·Dilson E RassierMaria Angela Bagni
Sep 2, 1998·Journal of Structural Biology·M S KellermayerH L Granzier
May 29, 2016·Journal of Biomechanics·Tobias SiebertNorman Stutzig
Apr 3, 2002·The Journal of Physiology·Ave MinajevaWolfgang A Linke
May 11, 2011·The Journal of Cell Biology·Katharina da Silva LopesMichael Gotthardt
Dec 29, 2016·The Journal of Physiology·Nabil ShalabiDilson E Rassier
Apr 8, 2009·Journal of Theoretical Biology·Urs StoeckerJachen Denoth
May 17, 2018·American Journal of Physiology. Cell Physiology·V JoumaaW Herzog
Jan 30, 2004·American Journal of Physiology. Cell Physiology·M A BagniG Cecchi
Oct 4, 2017·The Journal of Experimental Biology·Michael M DuVallWalter Herzog
Dec 21, 2016·The Journal of Experimental Biology·Jenna A MonroyKiisa C Nishikawa
Mar 6, 2016·The Journal of Experimental Biology·Krysta PowersWalter Herzog
Jan 23, 2016·The Journal of Experimental Biology·Barbara ColombiniMaria Angela Bagni
Jan 23, 2016·The Journal of Experimental Biology·Kiisa Nishikawa
Jun 24, 2017·The Journal of Experimental Biology·Krysta PowersWalter Herzog
Jul 10, 1998·FEBS Letters·C AstierY Benyamin
Dec 7, 2018·Journal of Applied Physiology·Johanna K Freundt, Wolfgang A Linke
Jul 9, 1999·Reviews of Physiology, Biochemistry and Pharmacology·R Horowits
May 29, 2007·Journal of Muscle Research and Cell Motility·Ivo A Telley, Jachen Denoth
Apr 3, 2019·Journal of Experimental Zoology. Part A, Ecological and Integrative Physiology·Natalie C Holt
Nov 11, 2011·Journal of Muscle Research and Cell Motility·Marta NocellaGiovanni Cecchi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.