Calcium-dependent protein kinase isoforms in Petunia have distinct functions in pollen tube growth, including regulating polarity

The Plant Cell
Gyeong Mee YoonAndrew G McCubbin

Abstract

Calcium is a key regulator of pollen tube growth, but little is known concerning the downstream components of the signaling pathways involved. We identified two pollen-expressed calmodulin-like domain protein kinases from Petunia inflata, CALMODULIN-LIKE DOMAIN PROTEIN KINASE1 (Pi CDPK1) and Pi CDPK2. Transient overexpression or expression of catalytically modified Pi CDPK1 disrupted pollen tube growth polarity, whereas expression of Pi CDPK2 constructs inhibited tube growth but not polarity. Pi CDPK1 exhibited plasma membrane localization most likely mediated by acylation, and we present evidence that suggests this localization is critical to the biological function of this kinase. Pi CDPK2 substantially localized to as yet unidentified internal membrane compartments, and this localization was again, at least partially, mediated by acylation. In contrast with Pi CDPK1, altering the localization of Pi CDPK2 did not noticeably alter the effect of overexpressing this isoform on pollen tube growth. Ca(2+) requirements for Pi CDPK1 activation correlated closely with Ca(2+) concentrations measured in the growth zone at the pollen tube apex. Interestingly, loss of polarity associated with overexpression of Pi CDPK1 was associated wit...Continue Reading

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Citations

Aug 2, 2013·Plant Cell Reports·Chun-Hai Dong, Yan Hong
Dec 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·An YanZhen-Biao Yang
Apr 23, 2011·Journal of Experimental Botany·Bárbara TavaresAna Bicho
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