Calcium-dependent stimulation of estrogen secretion by FSH from theca cells of the domestic hen (Gallus domesticus)

General and Comparative Endocrinology
O M Onagbesan, M J Peddie

Abstract

There is little information about the stimulation of estrogen secretion from theca cells of the domestic hen by follicle-stimulating hormone (FSH), and the mechanism of action of FSH through calcium has not been considered previously. The theca interna and externa cells from the third (F3) and fourth (F4) largest ovarian follicles of hens were separated, dispersed, and incubated in M199 with FSH (0.5 micrograms ml-1) or A23187 (0.1 or 1 microgram ml-1) with or without ethylene glycol bis(beta-aminoethyl ether)N,N'-tetraacetic acid (EGTA) (2 mM) in calcium-replete or in calcium-free M199. Alternatively, androstenedione (10(-6) M) was added to the cells as aromatizable substrate, with or without FSH and/or EGTA. Estradiol and estrone secreted into the media during a 4-hr incubation period were measured by RIA. FSH stimulated estradiol and estrone secretion from all the cell preparations. The effect of FSH was abolished by the addition of EGTA or in calcium-free medium. A23187 stimulated estradiol and estrone secretion by the same extent as FSH, but did not do so in calcium-free medium. Androstenedione enhanced estradiol and estrone secretion, but estrogen secretion was further raised by the simultaneous addition of FSH. This acti...Continue Reading

References

Nov 1, 1988·General and Comparative Endocrinology·O M Onagbesan, M J Peddie
Dec 1, 1986·Biology of Reproduction·F E Robinson, R J Etches
Mar 1, 1987·General and Comparative Endocrinology·C J JoynerT G Taylor
Aug 1, 1970·General and Comparative Endocrinology·R J Boucek, K Savard
Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen

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Citations

Dec 18, 2001·Brain Research. Brain Research Reviews·P AbsilJ Balthazart
Feb 19, 2002·The Journal of Steroid Biochemistry and Molecular Biology·J BalthazartG F Ball
Nov 19, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Jacques BalthazartGregory F Ball
May 22, 2009·Annals of the New York Academy of Sciences·Jacques BalthazartCharlotte A Cornil
Dec 20, 2005·Neuroscience·J BalthazartG F Ball
Oct 19, 2004·Physiology & Behavior·Jacques BalthazartGregory F Ball
Apr 9, 2015·Frontiers in Neuroscience·Thierry D CharlierJacques Balthazart

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