Calcium-dependent, sustained enhancement of excitability during washout of aconitine in rat hippocampal slices

Experimental Brain Research
A Ameri, T Peters

Abstract

Extracellular recording of the stimulus-evoked population spike was performed in the CA1 area of rat hippocampal slices in order to investigate delayed effects of the plant alkaloids aconitine and veratridine. Veratridine (1 microM and 10 microM) suppressed the orthodromic and antidromic population spike. After washout of the drug, only a partial recovery was obtained. Aconitine (1 microM) exerted the same inhibitory action as veratridine. However, after washout, the spike amplitude was enhanced compared with the control. This enhancement of the spike amplitude was dependent on the concentration of aconitine and was maintained during the observation period of at least 2 h. Lowering the Ca2+ concentration of the bathing medium from 2.5 mM to 1.25 mM during application of aconitine attenuated recovery and prevented the enhancement observed during washout of the drug. Application of aconitine in the presence of CdCl2 as well as in the presence of inhibitors of protein kinase C and Ca2+/calmodulin-dependent protein kinase II prevented the increase in spike amplitude during washout with standard artificial cerebrospinal fluid. In contrast, the N-methyl-D-aspartate (NMDA) receptor antagonists D-AP5 and MK-801 as well as the non-NMDA ...Continue Reading

Citations

Jan 22, 2010·Archives of Pharmacal Research·Cheng PengDing-Kun Zhang
Oct 7, 1998·Progress in Neurobiology·A Ameri
Feb 9, 2002·British Journal of Pharmacology·Hisashi YamanakaNorio Akaike
Jan 15, 2016·The Journal of Pain : Official Journal of the American Pain Society·Teng-Fei LiYong-Xiang Wang
Mar 11, 2008·European Journal of Pharmacology·Logan J VossJames W Sleigh

❮ Previous
Next ❯

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.