PMID: 11929611Apr 4, 2002Paper

Calcium-dependent transferrin receptor recycling in bovine chromaffin cells

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D E Knight

Abstract

The release of regulated secretory granules is known to be calcium dependent. To examine the Ca2+-dependence of other exocytic fusion events, transferrin recycling in bovine chromaffin cells was examined. Internalised 125I-transferrin was released constitutively from cells with a half-time of about 7 min. Secretagogues that triggered catecholamine secretion doubled the rate of 125I-transferrin release, the time courses of the two triggered secretory responses being similar. The triggered 125I-transferrin release came from recycling endosomes rather than from sorting endosomes or a triggered secretory vesicle pool. Triggered 125I-transferrin release, like catecholamine secretion from the same cells, was calcium dependent but the affinities for calcium were very different. The extracellular calcium concentrations that gave rise to half-maximal evoked secretion were 0.1 mm for 125I-transferrin and 1.0 mm for catecholamine, and the intracellular concentrations were 0.1 microm and 1 microm, respectively. There was significant 125I-transferrin recycling in the virtual absence of intracellular Ca2+, but the rate increased when Ca2+ was raised above 1 nm, and peaked at 1 microm when the rate had doubled. Botulinum toxin type D blocked ...Continue Reading

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Citations

Oct 27, 2004·Pharmacology & Therapeutics·Haley E Melikian
Mar 20, 2010·Nature Reviews. Neuroscience·Nicola B Hamilton, David Attwell
Mar 18, 2003·The Journal of Biological Chemistry·Ariel SavinaMaria I Colombo
Dec 14, 2007·The Journal of Biological Chemistry·Stan F J van de GraafVolker Gerke
May 19, 2019·The Journal of Pharmacology and Experimental Therapeutics·Rachel L MantheSilvia Muro
May 29, 2020·Cell Communication and Signaling : CCS·Mohammad Golam Sabbir
Apr 6, 2005·The Journal of Law, Medicine & Ethics : a Journal of the American Society of Law, Medicine & Ethics·Mayelin Prieto-Gonzalez
Nov 2, 2006·Journal of Cell Science·Sestina FalconeJacopo Meldolesi
Sep 28, 2004·Science·Mikyoung ParkMichael D Ehlers

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