Calcium-dependent upregulation of E4BP4 expression correlates with glucocorticoid-evoked apoptosis of human leukemic CEM cells.

Biochemical and Biophysical Research Communications
Saul J PricemanRheem D Medh

Abstract

Glucocorticoid (GC)-evoked apoptosis of T-lymphoid cells is preceded by increases in the intracellular Ca2+ concentration ([Ca2+]i), which may contribute to apoptosis. This report demonstrates that GC-mediated upregulation of the bZIP transcriptional repressor gene, E4BP4, is dependent on [Ca2+]i levels, and correlates with GC-evoked apoptosis of GC-sensitive CEM-C7-14 cells. Calcium chelators EGTA and BAPTA reduced [Ca2+]i levels and protected CEM-C7-14 cells from Dex-evoked E4BP4 upregulation as well as apoptosis. In the GC-resistant sister clone, CEM-C1-15, Dex treatment did not induce [Ca2+]i levels, E4BP4 expression or apoptosis, however, the calcium ionophore A23187 restored Dex-evoked E4BP4 upregulation and apoptosis. CEM-C7-14 cells were more sensitive to GC-independent increases in [Ca2+]i levels by thapsigargin, and a corresponding increase in E4BP4 expression and cell death, compared to CEM-C1-15 cells, suggesting a direct correlation between [Ca2+]i levels, E4BP4 expression, and apoptosis.

References

Feb 15, 1989·Archives of Biochemistry and Biophysics·D J McConkeyS Orrenius
Jun 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·R B Evans-Storms, J A Cidlowski
Dec 27, 1995·Biochimica Et Biophysica Acta·W J ChenJ G Gray
Jan 1, 1997·Critical Reviews in Eukaryotic Gene Expression·B M Jehn, B A Osborne
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·S IkushimaA T Look
Mar 17, 1997·Biochemical and Biophysical Research Communications·A D WallaceD A Young
Jun 30, 2000·Methods : a Companion to Methods in Enzymology·S W BurchielL A Sklar
Jan 5, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jerome GalonStefan R Bornstein
Oct 19, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Ian G Cowell
May 14, 2003·The Journal of Biological Chemistry·Ibrahim C Ozkurt, Sotirios Tetradis
Jul 2, 2003·Development·Sébastien SzuplewskiRégine Terracol
Aug 29, 2003·The Journal of Steroid Biochemistry and Molecular Biology·M Scott WebbE Brad Thompson
Apr 30, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Christian PlonerReinhard Kofler

❮ Previous
Next ❯

Citations

May 1, 2010·BMC Molecular Biology·Yasuko HirakawaStan Metzenberg
Jan 24, 2013·Journal of Autoimmunity·Ming ZhaoQianjin Lu
Oct 7, 2011·Journal of Molecular Signaling·Jessica A BeachRheem D Medh
Sep 4, 2009·Journal of Molecular Signaling·Yasuko HirakawaRheem D Medh
Jun 2, 2018·Journal of Cellular Physiology·Bharath Kumar VelmuruganChih-Yang Huang
Sep 26, 2015·Oncotarget·Ronit Vogt SionovZvi Granot
Oct 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Franco SilvestrisFranco Dammacco

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis