Abstract
Glucocorticoid (GC)-evoked apoptosis of T-lymphoid cells is preceded by increases in the intracellular Ca2+ concentration ([Ca2+]i), which may contribute to apoptosis. This report demonstrates that GC-mediated upregulation of the bZIP transcriptional repressor gene, E4BP4, is dependent on [Ca2+]i levels, and correlates with GC-evoked apoptosis of GC-sensitive CEM-C7-14 cells. Calcium chelators EGTA and BAPTA reduced [Ca2+]i levels and protected CEM-C7-14 cells from Dex-evoked E4BP4 upregulation as well as apoptosis. In the GC-resistant sister clone, CEM-C1-15, Dex treatment did not induce [Ca2+]i levels, E4BP4 expression or apoptosis, however, the calcium ionophore A23187 restored Dex-evoked E4BP4 upregulation and apoptosis. CEM-C7-14 cells were more sensitive to GC-independent increases in [Ca2+]i levels by thapsigargin, and a corresponding increase in E4BP4 expression and cell death, compared to CEM-C1-15 cells, suggesting a direct correlation between [Ca2+]i levels, E4BP4 expression, and apoptosis.
References
Feb 15, 1989·Archives of Biochemistry and Biophysics·D J McConkeyS Orrenius
Jan 1, 1984·The Journal of Pathology·A H WyllieD Dunlop
Jun 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·R B Evans-Storms, J A Cidlowski
Apr 1, 1995·Journal of Cellular Physiology·F Adebodun, J F Post
Jan 11, 1994·Nucleic Acids Research·I G Cowell, H C Hurst
Dec 27, 1995·Biochimica Et Biophysica Acta·W J ChenJ G Gray
Aug 8, 1996·Nature·M M MetzsteinH R Horvitz
Jan 1, 1997·Critical Reviews in Eukaryotic Gene Expression·B M Jehn, B A Osborne
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·S IkushimaA T Look
Mar 17, 1997·Biochemical and Biophysical Research Communications·A D WallaceD A Young
Oct 13, 1999·Molecular Cell·T InukaiA T Look
Jun 30, 2000·Methods : a Companion to Methods in Enzymology·S W BurchielL A Sklar
Mar 23, 2001·The Journal of Biological Chemistry·Y Nishimura, T Tanaka
Aug 9, 2001·Oncogene·M Unoki, Y Nakamura
Aug 11, 2001·Oncogene·R D MedhE B Thompson
Jan 5, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jerome GalonStefan R Bornstein
Oct 19, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Ian G Cowell
May 14, 2003·The Journal of Biological Chemistry·Ibrahim C Ozkurt, Sotirios Tetradis
Jun 5, 2003·Genomics·Rheem D MedhE Brad Thompson
Jul 2, 2003·Development·Sébastien SzuplewskiRégine Terracol
Aug 29, 2003·The Journal of Steroid Biochemistry and Molecular Biology·M Scott WebbE Brad Thompson
Apr 1, 1965·Cancer·G E FOLEYR E MCCARTHY
Jan 27, 2004·Transplantation·David C TongJames A Tumlin
Aug 13, 2004·Development·Dirk JunghansChristopher E Henderson
Apr 30, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Christian PlonerReinhard Kofler
Citations
Sep 15, 2009·Nature Immunology·Duncan M GascoyneHugh J M Brady
May 1, 2010·BMC Molecular Biology·Yasuko HirakawaStan Metzenberg
Nov 8, 2007·Bioscience, Biotechnology, and Biochemistry·Noriko KajitaniKozo Utsumi
Aug 8, 2014·Biochemical and Biophysical Research Communications·Jessica A BeachRheem D Medh
Jan 24, 2013·Journal of Autoimmunity·Ming ZhaoQianjin Lu
Oct 7, 2011·Journal of Molecular Signaling·Jessica A BeachRheem D Medh
Sep 4, 2009·Journal of Molecular Signaling·Yasuko HirakawaRheem D Medh
Jun 2, 2018·Journal of Cellular Physiology·Bharath Kumar VelmuruganChih-Yang Huang
Jan 10, 2019·Molecular Pharmacology·Miki MatsuiSusumu Ohya
Apr 21, 2017·Oncotarget·Souleymane Abdoul-AzizeJean-Pierre Vannier
Feb 22, 2013·Endocrinology·Kirstyn T CareyTimothy J Cole
Sep 26, 2015·Oncotarget·Ronit Vogt SionovZvi Granot
Oct 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Franco SilvestrisFranco Dammacco