Calcium domains around single and clustered IP3 receptors and their modulation by buffers.

Biophysical Journal
Sten RüdigerJ W Shuai

Abstract

We study Ca(2+) release through single and clustered IP(3) receptor channels on the ER membrane under presence of buffer proteins. Our computational scheme couples reaction-diffusion equations and a Markovian channel model and allows our investigating the effects of buffer proteins on local calcium concentrations and channel gating. We find transient and stationary elevations of calcium concentrations around active channels and show how they determine release amplitude. Transient calcium domains occur after closing of isolated channels and constitute an important part of the channel's feedback. They cause repeated openings (bursts) and mediate increased release due to Ca(2+) buffering by immobile proteins. Stationary domains occur during prolonged activity of clustered channels, where the spatial proximity of IP(3)Rs produces a distinct [Ca(2+)] scale (0.5-10 microM), which is smaller than channel pore concentrations (>100 microM) but larger than transient levels. While immobile buffer affects transient levels only, mobile buffers in general reduce both transient and stationary domains, giving rise to Ca(2+) evacuation and biphasic modulation of release amplitude. Our findings explain recent experiments in oocytes and provide a...Continue Reading

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Citations

Oct 19, 2012·Journal of Mathematical Biology·Je-Chiang Tsai
Jun 14, 2012·PLoS Computational Biology·Sten RüdigerJian-Wei Shuai
Apr 26, 2013·The Journal of Chemical Physics·Mark B FleggRadek Erban
Jun 21, 2012·Journal of Theoretical Biology·Gregory A Handy, Bradford E Peercy
Aug 24, 2016·Proceedings of the National Academy of Sciences of the United States of America·Brendan A Bicknell, Geoffrey J Goodhill
Nov 17, 2016·The European Physical Journal. E, Soft Matter·M Rückl, S Rüdiger
Dec 14, 2016·PLoS Computational Biology·James C SchaffBoris M Slepchenko
Jan 31, 2014·Physical Biology·Daniel Fraiman, Silvina Ponce Dawson
Apr 27, 2011·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Oriol Vallès-CodinaLutz Schimansky-Geier
Jul 16, 2014·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·S Rüdiger

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