Calcium electroporation in three cell lines: a comparison of bleomycin and calcium, calcium compounds, and pulsing conditions

Biochimica Et Biophysica Acta
Stine Krog FrandsenJ Gehl

Abstract

Electroporation with calcium (calcium electroporation) can induce ATP depletion-associated cellular death. In the clinical setting, the cytotoxic drug bleomycin is currently used with electroporation (electrochemotherapy) for palliative treatment of tumors. Calcium electroporation offers several advantages over standard treatment options: calcium is inexpensive and may readily be applied without special precautions, as is the case with cytostatic drugs. Therefore, details on the use of calcium electroporation are essential for carrying out clinical trials comparing calcium electroporation and electrochemotherapy. The effects of calcium electroporation and bleomycin electroporation (alone or in combination) were compared in three different cell lines (DC-3F, transformed Chinese hamster lung fibroblast; K-562, human leukemia; and murine Lewis Lung Carcinoma). Furthermore, the effects of electrical pulsing parameters and calcium compound on treatment efficacy were determined. Electroporation with either calcium or bleomycin significantly reduced cell survival (p<0.0001), without evidence of a synergistic effect. Cellular death following calcium or bleomycin treatment occurred at similar applied voltages, suggesting that similar pa...Continue Reading

References

Jul 1, 1996·General Pharmacology·L M MirS Orlowski
Sep 22, 1998·Nature Biotechnology·H Aihara, J Miyazaki
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·L M MirD Scherman
May 24, 2000·Cellular and Molecular Life Sciences : CMLS·M Brini, E Carafoli
Jun 1, 2000·Anti-cancer Drugs·M J JaroszeskiR Heller
May 24, 2001·Critical Reviews in Biochemistry and Molecular Biology·E CarafoliM Brini
Dec 25, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ruxandra Draghia-AkliRobert H Carpenter
Jul 3, 2003·Nature Reviews. Molecular Cell Biology·Michael J BerridgeH Llewelyn Roderick
Mar 14, 2008·Current Molecular Medicine·Carlotta GiorgiRosario Rizzuto
Apr 15, 2008·Bioelectrochemistry·P Thomas VernierGale L Craviso
May 24, 2008·Annals of Surgical Oncology·P QuaglinoM G Bernengo
Jun 10, 2008·Journal of Veterinary Internal Medicine·M CemazarJ Teissie
Oct 16, 2008·Anti-cancer Agents in Medicinal Chemistry·A Bergner, R M Huber
Nov 26, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Adil I DaudRichard Heller
May 26, 2009·Neurotoxicology·Ana-Maria Florea, Dietrich Büsselberg
Jul 28, 2009·Annals of Oncology : Official Journal of the European Society for Medical Oncology·A TestoriA M M Eggermont
Mar 20, 2010·International Journal of Cell Biology·Claudia CerellaLina Ghibelli
Apr 5, 2011·Cell Calcium·Boris Zhivotovsky, Sten Orrenius
Apr 8, 2011·Human Gene Therapy·Pernille HojmanHanne Gissel
May 18, 2011·Acta Oncologica·Louise Wichmann MatthiessenJulie Gehl
Aug 9, 2011·Annals of Surgical Oncology·Pietro CuratoloMaria Grazia Bernengo
Aug 23, 2011·Cancer Treatment Reviews·Gregor SersaMarko Snoj
Sep 8, 2011·Technology in Cancer Research & Treatment·I EdhemovicG Sersa
Apr 18, 2012·The British Journal of Surgery·L G CampanaC R Rossi
Jun 27, 2012·Acta Oncologica·Louise Wichmann MatthiessenJulie Gehl
Jul 10, 2012·Biochemical and Biophysical Research Communications·Richard NuccitelliJean Y Tang
Nov 23, 2012·Journal of Translational Medicine·Darja PavlinNatasa Tozon

❮ Previous
Next ❯

Citations

Jun 17, 2015·The Journal of Membrane Biology·Baltramiejus JakštysSaulius Šatkauskas
Mar 14, 2014·Biomedical Engineering Online·Damijan MiklavčičGregor Serša
Jul 28, 2016·PloS One·Janja DermolDamijan Miklavčič
Nov 17, 2017·The Journal of Membrane Biology·Violeta RajeckaitėSaulius Šatkauskas
Aug 22, 2017·The Journal of Membrane Biology·Florin CiobanuJustin Teissié
Jul 20, 2018·Technology in Cancer Research & Treatment·Stefania RomeoOlga Zeni
Sep 21, 2018·Technology in Cancer Research & Treatment·Daniel C SweeneyRafael V Davalos
Sep 22, 2018·Technology in Cancer Research & Treatment·Daniel C SweeneyRafael V Davalos
Jun 22, 2018·Scientific Reports·Barbara StaresinicMaja Cemazar
Mar 12, 2020·Applied Biochemistry and Biotechnology·Hong Bae KimKu Youn Baik
Aug 2, 2017·Cancer Research·Stine K FrandsenJulie Gehl
Nov 25, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Aleksander KiełbikJulita Kulbacka
Aug 24, 2021·Bioelectrochemistry·Maura B BendixPatrick F Forde
Dec 1, 2019·Bioelectricity·Maja Cemazar, Gregor Sersa
Apr 1, 2021·Dermatology : International Journal for Clinical and Investigative Dermatology·Hanne FalkJulie Gehl

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society
G SersaMarko Snoj
Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Marina N TorreroShulin Li
Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons
S OmuraK Fujita
Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete
Uwe Reinhold
© 2021 Meta ULC. All rights reserved