PMID: 7541085Mar 1, 1995Paper

Calcium entry in rabbit corneal epithelial cells: evidence for a nonvoltage dependent pathway

The Journal of Membrane Biology
A Rich, J L Rae

Abstract

We performed experiments to elucidate the calcium influx pathways in freshly dispersed rabbit corneal epithelial cells. Three possible pathways were considered: voltage-gated Ca++ channels, Na+/Ca++ exchange, and nonvoltage-dependent Ca(++)-permeable channels. Whole cell inward currents carrying either Ca++ or Ba++ were not detected using voltage clamp techniques. We also used imaging technology and the Ca(++)-sensitive ratiometric dye fura 2 to measure changes in intracellular Ca++ concentration ([Ca]i). Bath perfusion with NaCl Ringer's solution containing the calcium channel agonist Bay-K-8644 (1 microM), or Ni++ (40 microM), a blocker of many voltage-dependent calcium channels, did not affect [Ca++]i. Membrane depolarization with a KCl Ringer's bath solution resulted in a decrease in [Ca++]i. These results are inconsistent with the presence of voltage gated Ca++ channels. Nonvoltage gated Ca++ entry, on the other hand, would be reduced by membrane depolarization and enhanced by membrane hyperpolarization. Agents which hyperpolarize via stimulation of K+ current, such as flufenamic acid, resulted in an increase in ratio intensity. The cells were found to be permeable to Mn++ and bath perfusion with 5 mM Ni++ decreased [Ca++]...Continue Reading

Citations

Apr 15, 1999·Annals of Nuclear Medicine·A S ArbabT Araki
Mar 21, 1998·Kidney International·S M PetersR J Bindels
Mar 13, 2016·Survey of Ophthalmology·William StevensonMingwu Wang
Apr 21, 2007·Progress in Retinal and Eye Research·Stefan Mergler, Uwe Pleyer
Oct 25, 2011·Progress in Retinal and Eye Research·Min ZhaoBrian Reid
Jun 1, 1997·Experimental Eye Research·S I WatanabeA Kaneko
Sep 12, 2006·Progress in Retinal and Eye Research·Luo Lu
Sep 27, 2001·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·K Y WuH Z Wang
Mar 22, 2003·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Show-Jen HongJim C Fong
Sep 30, 2000·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·B Shi, R R Isseroff
Sep 2, 1999·Experimental Cell Research·J Y ParkC H Chung
Jan 29, 2002·Biochemical and Biophysical Research Communications·Jae-Yong ParkKyungjin Kim
Jun 1, 1997·The Journal of Investigative Dermatology·T MauroP A Pappone

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