PMID: 8600114Sep 1, 1995Paper

Calcium homeostasis in guinea pig type-I vestibular hair cell: possible involvement of an Na(+)-Ca2+ exchanger

Hearing Research
C ChabbertJ Lehouelleur

Abstract

In type-I vestibular hair cells (VHCs), the mechanisms involved in intracellular calcium homeostasis have not yet been established. In order to investigate the involvement of an Na(+)-dependent ionic exchanger in the regulation of cytosolic free calcium concentration, we analyzed the effect of the removal of external sodium on the cytosolic concentration of calcium ions ([Ca2+]i), sodium ions ([Na+]i), and protons (pHi). These concentrations were measured in type-I VHCs isolated from guinea pig labyrinth, using Fura-2, sodium benzofuran isophtalate (SBFI), and 1,4 diacetoxy-2,3 dicyanobenzol (ADB) respectively. Complete replacement of Na+ in the superfusion solution with N-methyl-D-glucamine (NMDG+), reversibly increased [Ca2+]i by 276 +/- 89% (n = 46) and decreased [Na+]i by 23 +/- 6% (n = 14). Both responses were prevented by removing external Ca2+ or chelating internal Ca2+. This suggests the presence of coupled Ca2+ and Na+ transport. The [Ca2+]i increase evoked by Na(+)-free solution was reduced by about 55% with the application of amiloride derivatives and was totally abolished in the presence of high [Mg2+]o. No pHi variation was detected during [Na+]o reduction. In the absence of external K+, the Na(+)-free solution fai...Continue Reading

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Citations

Feb 29, 2000·Biophysical Journal·M MartiniG Rispoli
May 21, 1999·The European Journal of Neuroscience·C BoyerJ Lehouelleur
Nov 15, 2002·The European Journal of Neuroscience·M MartiniG Rispoli
Nov 6, 2007·Hearing Research·Greg A O'Beirne, Robert B Patuzzi
Aug 10, 2018·Journal of Ophthalmic & Vision Research·Mohammad Mehdi Parvaresh
Jan 22, 1998·The American Journal of Physiology·K J RennieM J Correia

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