PMID: 7932239Jun 15, 1994Paper

Calcium homeostasis in identified rat gonadotrophs

The Journal of Physiology
A TseB Hille

Abstract

1. Whole-cell voltage clamp was used in conjunction with the fluorescent Ca2+ indicator indo-1 to measure extracellular Ca2+ entry and intracellular Ca2+ concentrations ([Ca2+]i) in rat gonadotrophs identified with the reverse haemolytic plaque assay. 2. Depolarizations to potentials more positive than -40 mV elicited inward Ca2+ current (ICa) and transient elevations of [Ca2+]i. 3. The relationship between [Ca2+]i elevations and Ca2+ entry with different Ca2+ buffer concentrations in the pipette showed that endogenous Ca2+ buffers normally bind approximately 99% of the Ca2+ entering the cell. 4. With [Ca2+]i elevations less than 500 nM, decay of [Ca2+]i could be approximated by an exponential whose time constant increased with the concentration of exogenous Ca2+ buffers. 5. Inhibitors of intracellular Ca(2+)-ATPases, thapsigargin, cyclopiazonic acid (CPA) and 2,5-di-(tert-butyl)-1,4-benzohydroquinone (BHQ), caused [Ca2+]i to rise. Application of BHQ during [Ca2+]i oscillations induced by gonadotrophin-releasing hormone (GnRH) terminated the oscillation in a slowly decaying elevation. BHQ slowed the decay of depolarization-induced [Ca2+]i elevations about 3-fold. 6. Taking into account the Ca2+ buffering properties of the cytop...Continue Reading

Citations

Nov 1, 1995·Neuropharmacology·E Neher
Dec 1, 1996·Trends in Endocrinology and Metabolism : TEM·S S Stojilkovic, M Tomić
Jun 5, 2001·The Journal of Physiology·A K Lee, A Tse
Dec 24, 1997·The Journal of Physiology·P Thomas, D W Waring
May 23, 2007·Aging Cell·David Murchison, William H Griffith
Jan 11, 2011·Synapse·Tao He, Gregory A Lnenicka
Dec 17, 2011·Cell Calcium·David J HodsonPatrice Mollard
Jan 10, 2012·Cell Calcium·Amy TseFrederick W Tse
Dec 6, 2011·Cell Calcium·Stanko S Stojilkovic
Sep 16, 2000·Journal of Theoretical Biology·P R ShortenD J Wall
Sep 25, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·F W Tse, A Tse
Sep 1, 1995·Pflügers Archiv : European journal of physiology·A TseB Hille
Dec 22, 2005·The Journal of Biological Chemistry·Karin OsibowWolfgang F Graier
Jul 24, 2010·Endocrine Reviews·Stanko S StojilkovicRichard Bertram
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Nov 11, 2016·Journal of Neurophysiology·Michael W Country, Michael G Jonz
Jul 19, 2000·Journal of Neurophysiology·L S DoveW H Griffith
Sep 23, 2000·Behavioural Brain Research·W H GriffithD Murchison

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