Calcium imaging of CPG-evoked activity in efferent neurons of the stick insect

PloS One
Jens GoldammerJoachim Schmidt

Abstract

The stick insect is a well-established experimental animal to study the neural basis of walking. Here, we introduce a preparation that allows combining calcium imaging in efferent neurons with electrophysiological recordings of motor neuron activity in the stick insect thoracic nerve cord. The intracellular free calcium concentration in middle leg retractor coxae motor neurons and modulatory octopaminergic DUM neurons was monitored after backfilling lateral nerve nl5 that contains the axons of these neurons with the calcium indicator Oregon Green BAPTA-1. Rhythmic spike activity in retractor and protractor motor neurons was evoked by pharmacological activation of central pattern generating neuronal networks and recorded extracellularly from lateral nerves. A primary goal of this study was to investigate whether changes in the intracellular free calcium concentration observed in motor neurons during oscillatory activity depend on action potentials. We show that rhythmic spike activity in leg motor neurons induced either pharmacologically or by tactile stimulation of the animal is accompanied by a synchronous modulation in the intracellular free calcium concentration. Calcium oscillations in motor neurons do not appear to depend ...Continue Reading

References

Sep 1, 1975·The Journal of Experimental Zoology·G Hoyle
Feb 1, 1990·Cell Calcium·R Y Tsien, A T Harootunian
Jan 1, 1988·Comparative Biochemistry and Physiology. A, Comparative Physiology·J SchmitzF Delcomyn
Jul 1, 1973·The Journal of General Physiology·G R Strichartz
Mar 1, 1982·The Journal of Physiology·P D Evans, M V Siegler
May 1, 1993·Journal of Neurophysiology·S Ryckebusch, G Laurent
Nov 1, 1995·Current Biology : CB·D L BennettT R Cheek
Nov 1, 1996·Journal of Neurophysiology·R M Johnston, R B Levine
Mar 1, 1997·The Journal of Physiology·M J Berridge
Feb 21, 1998·Journal of Neurophysiology·S BaudouxO T Morris
Jun 26, 1998·Brain Research. Brain Research Reviews·U Bässler, A Büschges
Jan 15, 2000·Progress in Brain Research·H Hultborn
Dec 1, 2001·Current Biology : CB·E Marder, D Bucher
Mar 6, 2002·Journal of Neurophysiology·C Duch, R B Levine
Aug 22, 2003·Journal of Neurophysiology·Shin-ichi HigashijimaJoseph R Fetcho
Apr 14, 2004·The European Journal of Neuroscience·Ansgar BüschgesRalph A DiCaprio
Sep 25, 2007·Brain Research Reviews·Ansgar BüschgesJoachim Schmidt
Oct 13, 2007·Journal of Insect Physiology·Tim MentelHans-Joachim Pflüger
Dec 13, 2007·Physiology·Werner Göbel, Fritjof Helmchen
Mar 25, 2009·The European Journal of Neuroscience·Tom Baden, Berthold Hedwig
Jun 12, 2009·Journal of Neurophysiology·Sandra WestmarkJoachim Schmidt
Mar 20, 2010·Journal of Neurophysiology·Eugênio E OliveiraPeter Kloppenburg
Oct 26, 2010·Current Opinion in Neurobiology·David L McLean, Joseph R Fetcho
Dec 21, 2011·The Journal of Physiology·Stefanie RyglewskiCarsten Duch
Mar 13, 2012·Neuron·Christine Grienberger, Arthur Konnerth
May 2, 2012·Journal of Visualized Experiments : JoVE·Dvir Blivis, Michael J O'Donovan
Aug 31, 2012·Nature Reviews. Neuroscience·Thomas Knöpfel
Dec 19, 2012·Current Opinion in Neurobiology·Ruben PortuguesMisha B Ahrens
Jan 31, 2013·Current Pharmaceutical Design·Hans HultbornClaire F Meehan
Mar 28, 2013·Annals of the New York Academy of Sciences·Christopher A Hinckley, Samuel L Pfaff
Jul 19, 2013·Nature Communications·Xiaonan R SunSamuel S-H Wang

❮ Previous
Next ❯

Citations

Mar 5, 2020·Developmental Neurobiology·Charalampos MantziarisAnsgar Büschges

❮ Previous
Next ❯

Software Mentioned

Spike
AxioVision
Matlab
Excel
IgorPro
Corel Draw
SPIKE2

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.