Abstract
Although inhibitory postsynaptic currents (IPSCs) mediated by GABA(A) receptor is thought to be affected by intracellular calcium ion concentration ([Ca2+]i), origin or route of [Ca2+]i increment has not been well elucidated. Reports on the effect of [Ca2+]i elevation on GABA(A)ergic IPSCs per se are also controversial. In this study, effects of caffeine and several other [Ca2+]i-mobilizing drugs were examined on the IPSCs in acute slices of rat hippocampus. Using the patch clamp recording method, spontaneous and evoked currents were recorded from CA3 neurons. Caffeine strongly inhibited both extra-synaptic and synaptic GABAergic IPSCs, regardless of the presence or absence of extracellular Ca2+. This inhibition was not relieved by the intracellular application of EGTA or 1,2-bis(2-aminophenoxy)-ethane-N,N,N',N'-tetraacetic acid (BAPTA). This inhibition by caffeine was not prevented by preequilibration with caffeine. Ca2+ store depletion caused by thapsigargin or repetitive stimulation by caffeine could not prevent the inhibition. Moreover, ruthenium red and ryanodine could not overcome the inhibition. On the contrary, GABA(A)ergic currents were not inhibited by stimulation with several Ca2+-mobilizing agonists. Forskolin could...Continue Reading
References
Dec 5, 1989·European Journal of Pharmacology·F LopezR I Shader
Jun 1, 1993·British Journal of Pharmacology·H UneyamaN Akaike
Jan 15, 1995·The Journal of Physiology·Q X Chen, R K Wong
Oct 1, 1994·Neuroscience·A Stelzer, H Shi
Jan 1, 1996·Neuropharmacology·Y De Koninck, I Mody
Jul 1, 1997·The Journal of Physiology·O GaraschukA Konnerth
Feb 28, 1998·Pflügers Archiv : European journal of physiology·L G AguayoL S Satin
Mar 16, 2000·Neuroscience·M Taketo, T Yoshioka
Oct 24, 2000·The Journal of Comparative Neurology·E Y ChenJ H Kordower
Mar 10, 2001·Neuroreport·T SugimotoS Okada
Oct 24, 2002·Trends in Neurosciences·Jean-Luc GaiarsaYehezkel Ben-Ari
Jun 20, 2003·The European Journal of Neuroscience·Jozsef Vigh, Eric M Lasater
Citations
Sep 24, 2013·Neurochemistry International·Lisiane O PorciúnculaDenis B Rosemberg
Aug 5, 2010·Journal of Medicinal Chemistry·Tsuyoshi SakuradaRyuichi Sakai
Oct 29, 2013·Cellular and Molecular Life Sciences : CMLS·Julie A Mustard
Mar 22, 2016·Neural Plasticity·Masako Isokawa
Oct 13, 2009·Neuroscience·J-B KeZ Wang
Jul 25, 2009·Brain Research Bulletin·M Taketo, H Matsuda
Feb 19, 2008·Anaesthesia·M HüpflP Nagele
May 5, 2016·Human & Experimental Toxicology·A Aghabiklooei, J Sangsefidi
Feb 10, 2006·Journal of Neurophysiology·Masako Isokawa, Bradley E Alger
Jun 8, 2007·Journal of Neurophysiology·Zhi LiuTimothy H Murphy
Oct 18, 2005·The Journal of Pharmacology and Experimental Therapeutics·S L Cockerill, J S Mitcheson
Jul 1, 2017·Journal of Caffeine Research·Rachel L Poole, Michael G Tordoff
Nov 5, 2020·Neurochemistry International·Tina Hinton, Graham A R Johnston