PMID: 9188807May 22, 1997Paper

Calcium-induced bilirubin-dependent hemolysis of human erythrocytes

Biochimica Et Biophysica Acta
M K Ali, S Tayyab

Abstract

Human erythrocytes, preincubated with different concentrations of calcium chloride (0.17-1.67 mM) showed hemolysis after addition of bilirubin (72 microM). Hemolysis was observed only when cells were incubated first with calcium followed by bilirubin and not vice versa. This hemolysis was found to be dependent upon several factors such as concentration of bilirubin, time of incubation of erythrocytes with calcium and time of incubation of bilirubin with the calcium-loaded erythrocytes. Inclusion of EDTA in the incubation medium reduced the percentage hemolysis to a significant extent. Involvement of activated oxygen species in hemolytic process seems to be unlikely as inclusion of sodium azide and catalase did not prevent hemolysis. A comparison of other bivalent cations such as Ba2+, Mg2+, Mn2+ and Cu2+ with Ca2+ for their ability to hemolyse cells in presence of bilirubin shows that Ba2+ and Mg2+ are ineffective, whereas both Mn2+ and Cu2+ induce hemolysis both in the absence as well as in the presence of bilirubin. However, their mechanism of hemolysis is different from that of calcium-induced hemolysis. Formation of calcium-induced hydrophobic aggregates of phospholipid molecules in erythrocyte membrane may open the new bin...Continue Reading

References

Jun 5, 1973·Biochemistry·E J Shimshick, H M McConnell
Sep 1, 1974·Analytical Chemistry·J D Van Norman, R Szentirmay
Feb 1, 1972·Scandinavian Journal of Clinical and Laboratory Investigation·D Bratlid
Sep 30, 1980·Biochimica Et Biophysica Acta·Y Barenholz, T E Thompson

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Citations

Jan 1, 2001·Indian Journal of Clinical Biochemistry : IJCB·M K AliS Tayyab
Jan 1, 2008·Indian Journal of Clinical Biochemistry : IJCB·Boh Boon Kim, Saad Tayyab
Jan 10, 2004·The Journal of Laboratory and Clinical Medicine·Michael MarkWalter H Reinhart
Apr 6, 1999·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·M K Ali, S Tayyab
Jul 11, 2006·Journal of Biomedical Materials Research. Part a·S Suri, R Banerjee
Oct 3, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Shuiquan ZhangChangsheng Liu

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