Calcium-induced calcium release contributes to somatic secretion of serotonin in leech Retzius neurons

Journal of Neurobiology
Citlali TruetaFrancisco F De-Miguel

Abstract

We analyzed the contribution of calcium (Ca2+)-induced Ca2+ release to somatic secretion in serotonergic Retzius neurons of the leech. Somatic secretion was studied by the incorporation of fluorescent dye FM1-43 upon electrical stimulation with trains of 10 impulses and by electron microscopy. Quantification of secretion with FM1-43 was made in cultured neurons to improve optical resolution. Stimulation in the presence of FM1-43 produced a frequency-dependent number of fluorescent spots. While a 1-Hz train produced 19.5+/-5.0 spots/soma, a 10-Hz train produced 146.7+/-20.2 spots/soma. Incubation with caffeine (10 mM) to induce Ca2+ release from intracellular stores without electrical stimulation and external Ca2+, produced 168+/-21.7 spots/soma. This staining was reduced by 49% if neurons were preincubated with the Ca2+- ATPase inhibitor thapsigargin (200 nM). Moreover, in neurons stimulated at 10 Hz in the presence of ryanodine (100 microM) to block Ca2+-induced Ca2+ release, FM1-43 staining was reduced by 42%. In electron micrographs of neurons at rest or stimulated at 1 Hz in the ganglion, endoplasmic reticulum lay between clusters of dense core vesicles and the plasma membrane. In contrast, in neurons stimulated at 20 Hz, t...Continue Reading

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Citations

Aug 31, 2010·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Qin Li, Brian D Burrell
Jul 29, 2005·Cellular and Molecular Neurobiology·Francisco F De-Miguel, Citlali Trueta
May 12, 2010·Journal of Neurophysiology·Kathryn B Grey, Brian D Burrell
May 22, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jyoti C PatelMargaret E Rice
Mar 31, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Kevin M Crisp, Kenneth J Muller
Nov 2, 2005·Progress in Neurobiology·William B KristanW Otto Friesen
May 27, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Margaret E Rice, Jyoti C Patel
May 27, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Francisco F De-MiguelBruno Mendez
Jul 16, 2014·Frontiers in Cellular Neuroscience·Marco Canepari
Jul 16, 2014·Frontiers in Cellular Neuroscience·Carolina Leon-PinzonFrancisco F De-Miguel
Feb 26, 2020·Journal of Neuroscience Research·Oliva Mota SeguraMichael W Baker
Jan 9, 2019·Frontiers in Neuroscience·Emily QuentinLuc Maroteaux
Jun 26, 2018·Frontiers in Synaptic Neuroscience·Elaine Del-Bel, Francisco F De-Miguel
Jun 20, 2019·Frontiers in Physiology·Paula NoguezFrancisco F De-Miguel
Mar 27, 2021·BMC Genomics·Elizabeth Heath-HeckmanDavid Weisblat
May 18, 2021·Frontiers in Molecular Neuroscience·Francisco F De-MiguelJ Miguel Rubí

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