PMID: 11902111Mar 21, 2002Paper

Calcium-induced cytochrome c release from CNS mitochondria is associated with the permeability transition and rupture of the outer membrane

Journal of Neurochemistry
Nickolay BrustovetskyJanet M Dubinsky

Abstract

The mechanisms of Ca2+-induced release of Cytochrome c (Cyt c) from rat brain mitochondria were examined quantitatively using a capture ELISA. In 75 or 125 mm KCl-based media 1.4 micromol Ca2+/mg protein caused depolarization and mitochondrial swelling. However, this resulted in partial Cyt c release only in 75 mm KCl. The release was inhibited by Ru360, an inhibitor of the Ca2+ uniporter, and by cyclosporin A plus ADP, a combination of mitochondrial permeability transition inhibitors. Transmission electron microscopy (TEM) revealed that Ca2+-induced swelling caused rupture of the outer membrane only in 75 mm KCl. Koenig's polyanion, an inhibitor of mitochondrial porin (VDAC), enhanced swelling and amplified Cyt c release. Dextran T70 that is known to enhance mitochondrial contact site formation did not prevent Cyt c release. Exposure of cultured cortical neurons to 500 microM glutamate for 5 min caused Cyt c release into the cytosol 30 min after glutamate removal. MK-801 or CsA inhibited this release. Thus, the release of Cyt c from CNS mitochondria induced by Ca2+ in vitro as well as in situ involved the mPT and appeared to require the rupture of the outer membrane.

Citations

Dec 2, 2004·Journal of Neuroscience Research·P G SullivanJ E Springer
Mar 6, 2007·The Journal of Membrane Biology·Morena BurattaLanfranco Corazzi
Jan 23, 2008·Journal of Bioenergetics and Biomembranes·Marianna E JungJames W Simpkins
Mar 2, 2011·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Melanie L McEwenJoe E Springer
Oct 15, 2013·Acta neurologica Belgica·Jian ChenYuqi Gao
Oct 18, 2002·Neuroscience Letters·Nickolay BrustovetskyJanet M Dubinsky
Sep 16, 2010·Nature Reviews. Neurology·Catrina Sims-RobinsonEva L Feldman
Nov 13, 2001·Clinical and Experimental Pharmacology & Physiology·R VinkI Cernak
Dec 18, 2002·Journal of Neurochemistry·Nickolay BrustovetskyRonald Jemmerson
Oct 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Thomas W FaustPatricio T Huerta
Jun 17, 2010·The Journal of Biological Chemistry·Francesca FieniDavide Trotti
Oct 7, 2004·Journal of the American College of Nutrition·Mildred S SeeligBella T Altura
Aug 12, 2003·Journal of Neurotrauma·Jose L Perez VelazquezMarina V Frantseva
Aug 12, 2003·Journal of Neurotrauma·Alexander G RabchevskyStephen W Scheff
Aug 24, 2005·Antioxidants & Redox Signaling·Ronald JemmersonNickolay Brustovetsky
Aug 7, 2007·Antioxidants & Redox Signaling·Paula I MoreiraCatarina R Oliveira
Jul 30, 2009·Journal of Neurotrauma·Lesley K GilmerStephen W Scheff
Apr 28, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Hua NaranmanduraNoriyuki Suzuki
Mar 24, 2005·Journal of Cellular and Molecular Medicine·S Gupta, A A Knowlton
Mar 13, 2010·BMC Research Notes·Sabine EisenhoferHans Zischka
Mar 15, 2014·Disease Models & Mechanisms·Kelley C O'DonnellAlvaro Sagasti
Mar 18, 2006·Current Opinion in Critical Care·Amedeo Merenda, Ross Bullock
Dec 9, 2010·The International Journal of Neuroscience·Mohita ShrivastavaTaposh K Das
Apr 10, 2013·Journal of the Neurological Sciences·Daiva BironaiteAlgirdas Venalis
Nov 9, 2004·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Antonio SessoJoyce T Kawakami
Apr 24, 2012·Biochemical and Biophysical Research Communications·Fang XiaoCaigao Zhong
Jan 19, 2016·International Immunopharmacology·Lisa A RiesbergKristen M Drescher
May 25, 2011·Pharmacology & Therapeutics·Alexander G RabchevskyJoe E Springer
Jun 1, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M Van MoorhemL Leybaert
Mar 30, 2010·Molecular Aspects of Medicine·Varda Shoshan-BarmatzNir Arbel
Apr 28, 2009·Experimental Neurology·Aashish JoshiJames W Geddes

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