PMID: 2503513Aug 25, 1989Paper

Calcium induction of the mRNAs for prolactin and c-fos is independent of protein kinase C activity.

The Journal of Biological Chemistry
S K Bandyopadhyay, C Bancroft

Abstract

Ca2+ is a strong regulator of the expression of a limited number of eukaryotic genes, including prolactin and c-fos. However, little is known about the cellular signal transduction pathways involved in the action of this ion on specific gene expression. The Ca2+-dependent enzyme protein kinase C has been implicated in other transcriptional pathways regulating the prolactin and c-fos genes. We therefore employed down-regulation of protein kinase C in rat pituitary cells to investigate whether this enzyme is involved in Ca2+ regulation of expression of either of these genes. Exposure of Ca2+-deprived GH3 cells to this ion in the presence of the Ca2+ channel modulator Bay K8644 yielded large increases in the mRNAs for both prolactin and c-fos. Incubation of cells with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) yielded a 7- to 15-fold decrease in the enzymatic activity of C kinase assayed in vitro, but little or no effect on Ca2+ stimulation of cellular levels of either mRNA. The latter observation was apparently not due simply to an inability of TPA to down-regulate the gene-regulatory activity of kinase C in intact GH3 cells, since this phorbol ester blocked the stimulation by platelet-derived growth factor of c...Continue Reading

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