Calcium influx is not required for thyrotropin-releasing hormone stimulation of prolactin release from GH3 cells

Cell Calcium
M C GershengornR H Gerry

Abstract

TRH stimulation of prolactin release from GH3 cells is dependent on Ca2+; however, whether TRH-induced influx of extracellular Ca2+ is required for stimulated secretion remains controversial. We studied prolactin release from cells incubated in medium containing 110 mM K+ and 2 mM EGTA which abolished the electrical and Ca2+ concentration gradients that usually promote Ca2+ influx. TRH caused prolactin release and 45Ca2+ efflux from cells incubated under these conditions. In static incubations, TRH stimulated prolactin secretion from 11.4 +/- 1.2 to 19 +/- 1.8 ng/ml in control incubations and from 3.2 +/- 0.6 to 6.2 +/- 0.8 ng/ml from cells incubated in medium with 120 mM K+ and 2 mM EGTA. We conclude that Ca2+ influx is not required for TRH stimulation of prolactin release from GH3 cells.

References

Apr 28, 1978·Annals of the New York Academy of Sciences·E Carafoli, M Crompton
Apr 14, 1978·Biochemical and Biophysical Research Communications·A H TashjianD Maina
Oct 1, 1978·Endocrinology·R E OstlundM Melman
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S Ozawa, N Kimura
Nov 1, 1968·British Journal of Pharmacology·W W Douglas
May 12, 1977·Nature·B BialesA Tischler

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Citations

Sep 30, 1986·Biochemical and Biophysical Research Communications·G SnyderJ Capdevila
Feb 1, 1985·Metabolism: Clinical and Experimental·L TebaH C Taylor

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