Calcium influx pathways in rat CNS pericytes

Brain Research. Molecular Brain Research
Masahiro KamouchiMitsuo Iida

Abstract

In central nervous system (CNS), pericytes have been proposed to play a role in broad functional activities including blood-brain barrier, microcirculation, and macrophage activity. However, contractile responses and Ca2+ signaling in CNS pericytes have not been elucidated. The aim of the present study is to investigate contractility and Ca2+ influx pathway in CNS pericytes. CNS pericytes were cultured from rat brain. Contraction of the pericytes in response to various stimuli was evaluated by the change in surface area measured by a light microscope with a digital camera. Reverse transcription and polymerase chain reaction (RT-PCR) was performed to examine the expression of mRNA of alpha-smooth muscle actin. Intracellular Ca2+ was measured using fura-2 fluorescence spectroscopy. A23187 (Ca2+ ionophore), high external K+ (4 x 10(-2) mol/l), endothelin-1, and serotonin induced contraction of CNS pericytes. RT-PCR analysis revealed the expression of alpha-smooth muscle actin in CNS pericytes. Cytosolic Ca2+ ([Ca2+]i) increased after application of high concentration of external K+, tetraethylammonium, and charybdotoxin, which was inhibited by nicardipine and removal of external Ca2+. Angiotensin-II, serotonin, acetylcholine, ATP,...Continue Reading

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Citations

Jun 10, 2011·Acta Neuropathologica·Turgay DalkaraMuge Yemisci
Nov 10, 2010·Cellular and Molecular Neurobiology·Masahiro KamouchiTakanari Kitazono
Oct 28, 2011·Nature Neuroscience·Ethan A WinklerBerislav V Zlokovic
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Feb 22, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Frank M Faraci
Jan 22, 2009·Microvascular Research·Kuniyuki NakamuraMitsuo Iida
Mar 14, 2007·Neuroscience Letters·Masahiro KamouchiMitsuo Iida
Jun 20, 2014·Brain Pathology·Ethan A WinklerBerislav V Zlokovic
Oct 4, 2005·Brain Research. Brain Research Reviews·Char-Huei Lai, Kuo-Hsing Kuo
Apr 12, 2015·Brain Research·Turgay Dalkara, Luis Alarcon-Martinez
May 27, 2016·Nature Neuroscience·Melanie D SweeneyBerislav V Zlokovic
Jun 7, 2014·Journal of Vascular Research·Theodor Burdyga, Lyudmyla Borysova
May 31, 2018·Cell Transplantation·Jyoti Gautam, Yao Yao
Feb 12, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Kuniyuki NakamuraMitsuo Iida
Sep 29, 2019·Current Neuropharmacology·Andrew P CarlsonClaude W Shuttleworth
Dec 12, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Sofie HørlyckBirger Brodin
Jul 10, 2021·Frontiers in Neuroscience·Lu CaoWei Zhang

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