Calcium influx via TRP channels is required to maintain PIP2 levels in Drosophila photoreceptors

Neuron
R C HardieS T Sweeney

Abstract

The trp (transient receptor potential) gene encodes a Ca2+ channel responsible for the major component of the phospholipase C (PLC) mediated light response in Drosophila. In trp mutants, maintained light leads to response decay and temporary total loss of sensitivity (inactivation). Using genetically targeted PIP2-sensitive inward rectifier channels (Kir2.1) as biosensors, we provide evidence that trp decay reflects depletion of PIP2. Two independent mutations in the PIP2 recycling pathway (rdgB and cds) prevented recovery from inactivation. Abolishing Ca2+ influx in wild-type photoreceptors mimicked inactivation, while raising Ca2+ by blocking Na+/Ca2+ exchange prevented inactivation in trp. The results suggest that Ca2+ influx prevents PIP2 depletion by inhibiting PLC activity and facilitating PIP2 recycling. Without this feedback one photon appears sufficient to deplete the phosphoinositide pool of approximately 4 microvilli.

References

May 1, 1990·The Journal of General Physiology·P Hochstrate, K Hamdorf
Dec 1, 1989·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·P Hochstrate
Sep 22, 1987·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·J HowardS B Laughlin
Oct 18, 1969·Nature·D J Cosens, A Manning
Mar 1, 1982·The Journal of General Physiology·B Minke
Nov 7, 1995·Proceedings of the National Academy of Sciences of the United States of America·M A LemmonJ Schlessinger
May 26, 1995·The Journal of Biological Chemistry·J L Running DeerS L Yarfitz
Mar 1, 1994·The Journal of General Physiology·R C Hardie, B Minke
May 18, 1999·Cell Calcium·B Cook, B Minke
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·P G Alloway, P J Dolph
Dec 14, 1999·The Journal of Biological Chemistry·T RohácsD E Logothetis
Mar 16, 2000·Trends in Neurosciences·C HarteneckG Schultz
Apr 4, 2000·The Journal of Physiology·S R HendersonR C Hardie
May 12, 2000·Nature Cell Biology·B CookB Minke
May 17, 2000·Proceedings of the National Academy of Sciences of the United States of America·K M SullivanG M Rubin
Jun 2, 2000·Molecular and Cellular Neurosciences·P RaghuR C Hardie

❮ Previous
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Citations

Feb 14, 2012·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Kenji TomiokaTaishi Yoshii
Oct 13, 2006·Pflügers Archiv : European journal of physiology·Tibor Rohacs
May 10, 2007·Pflügers Archiv : European journal of physiology·Tao Wang, Craig Montell
May 8, 2008·Pflügers Archiv : European journal of physiology·Yuji KarashimaBernd Nilius
Feb 3, 2011·Pflügers Archiv : European journal of physiology·Roger C Hardie
Jun 1, 2005·Current Opinion in Neurobiology·Byung-Chang Suh, Bertil Hille
Aug 7, 2013·Advances in Biological Regulation·Shamshad Cockcroft, Kathryn Garner
Dec 31, 2003·Trends in Neurosciences·Patrick DelmasDavid A Brown
May 27, 2003·Cell Calcium·Roger C Hardie
Oct 24, 2001·Trends in Neurosciences·D Deitcher
Aug 6, 2003·Trends in Neurosciences·Stefan R NahorskiMark S Nash
Sep 9, 2010·Nature Reviews. Molecular Cell Biology·Sima Lev
Jul 22, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Konstantin NikolicChris Toumazou
Jul 24, 2012·The Journal of Biological Chemistry·Kathryn GarnerShamshad Cockcroft
Oct 19, 2005·The Journal of General Physiology·Jason LeeChou-Long Huang
Dec 28, 2006·The Journal of General Physiology·Moshe ParnasBaruch Minke
Oct 16, 2012·Science·Roger C Hardie, Kristian Franze
Feb 25, 2005·Science's STKE : Signal Transduction Knowledge Environment·Craig Montell
Feb 1, 2003·Annual Review of Physiology·Roger C Hardie
Feb 8, 2006·Annual Review of Physiology·Baruch Minke, Moshe Parnas
May 23, 2008·American Journal of Physiology. Cell Physiology·Donghee KimDina Simkin
Feb 5, 2013·Journal of Cell Science·Sukanya SenguptaRoger C Hardie
Jan 4, 2013·PloS One·Andrea SchneiderHenrike Scholz
Jun 7, 2011·Reviews in the Neurosciences·Claudio CoddouJ Pablo Huidobro-Toro
Dec 16, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Claudio VillalobosRodrigo Andrade
May 10, 2011·Annual Review of Biochemistry·Silvia Carrasco, Tobias Meyer
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Dan Liu, Emily R Liman
Jul 22, 2006·The Journal of Physiology·Yuichiro Fujiwara, Yoshihiro Kubo
Sep 23, 2006·The Journal of Physiology·Roger C Hardie
Aug 11, 2012·Life Sciences·Melissa A Fowler, Craig Montell
Aug 5, 2016·Communicative & Integrative Biology·Hanaa HaririW Mike Henne
Mar 1, 2016·Biochimica Et Biophysica Acta·Chi-Lun Chang, Jen Liou
Jan 11, 2007·Seminars in Cell & Developmental Biology·Padinjat Raghu
May 21, 2008·Current Opinion in Cell Biology·Giovanni D'AngeloMaria Antonietta De Matteis
Mar 31, 2007·The Journal of Physiology·Thomas Voets, Bernd Nilius
May 5, 2011·Developmental Neurobiology·Hongai Xia, Donald F Ready
Jun 30, 2006·Cell Calcium·Baruch Minke
May 15, 2013·Biophysical Journal·Brian ChuRoger C Hardie
Feb 1, 2011·Critical Reviews in Biochemistry and Molecular Biology·Shamshad Cockcroft, Kathryn Garner

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