PMID: 9639342Jun 25, 1998Paper

Calcium-inhibitable current in cultured embryonic chick cardiac myocytes: possibly via a novel chloride channel

Experimental Physiology
S J Liu, J R Stimers

Abstract

The role of extracellular Ca2+ (Ca(2+)o) in the modulation of cardiac Cl- currents (I(Cl)) such as those activated by cAMP or swelling is uncertain. The effects of Ca(2+)o and extracellular cadmium (Cd(2+)o) on Cl- currents in cultured chick cardiac myocytes were investigated in Na+- and K+-free internal and external solutions using the whole-cell patch-clamp technique. In the absence of Na+ and K+ internally and externally, the whole-cell current was predominantly I(Cl). In the absence of cAMP, removal of Ca(2+)o (+ 1 mM EGTA) resulted in an increase in the current that was suppressed by reduction of Cl(o)- with a rightward shift of the zero-current potential towards the CI- reversal potential. We designated this current as a Ca2+-inhibitable I(Cl). Addition of 0.5 mM Cd(2+)o with or without removal of Ca(2+)o also resulted in a 1.5- to 2.0-fold increase in I(Cl) that was attenuated by 1 mM DIDS (4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid). Under similar conditions, I(Cl) activated by Cd(2+)o (in 1 mM Ca(2+)o solution) was not further increased by subsequent removal of Ca(2+)o, suggesting that addition of Cd(2+)o and removal of Ca(2+)o activated the same I(Cl). In contrast, exposure to 1 microM forskolin further enhan...Continue Reading

Citations

Feb 10, 2016·Differentiation; Research in Biological Diversity·Tim P KelderMonique R M Jongbloed
Jun 14, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Yanfang XuNipavan Chiamvimonvat

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